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Rabbit Anti-DIO3 Polyclonal Antibody#abs138052

Please note that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis DIO3 using COS7 whole cell lysates This product is for research use only, not for use in diagnostic prodecures...

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Description

Catalog-specification	Delivery time	USD price
abs138052-100ug	1-2 Weeks	301.0
abs138052-50ug	1-2 Weeks	201.0

Please note that the mentioned price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs138052
Other names	Iodothyronine deiodinase type 3, also known as D3 or Dio3, is an enzyme involved in the regulation of thyroid hormone activity. It plays a crucial role in converting thyroxine (T4) into the biologically inactive form, reverse triiodothyronine (rT3). D3 is widely expressed in various tissues, including the placenta, where it is involved in regulating fetal thyroid hormone levels. The gene encoding D3 in humans is called DIOIII or IOD3_HUMAN, and it is also known by other names such as MGC124117 and MGC124118. D3 is classified as a selenoprotein, meaning it contains the essential trace element selenium. D3 is responsible for the conversion of T4 to rT3, which helps to regulate the overall thyroid hormone balance in the body. By converting T4 into rT3, D3 reduces the amount of active thyroid hormone available to cells. This process is particularly important during pregnancy, as it ensures that fetal thyroid hormone levels are tightly regulated. In summary, D3 is an enzyme that acts as a type III iodothyronine deiodinase. It plays a crucial role in regulating the levels of thyroid hormones, particularly during pregnancy. The accurate functioning of D3 is essential for maintaining thyroid hormone balance and ensuring proper development and growth.
Source	Rabbit
Specificity	The DIO3 Antibody has the ability to detect the total levels of endogenous DIO3 in the body. This means that it is capable of identifying all forms of the protein that exist naturally, without any interference or alteration. To ensure accuracy, it is important to use this antibody according to the recommended protocols and to handle the samples carefully to avoid degradation or contamination. With its high sensitivity and specificity, the DIO3 Antibody is a valuable tool for researchers studying thyroid hormone metabolism and related processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Dog;Bovine;
Antigen	DIO3
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human DIO3.
MW	31 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the purification process of the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	The product should be stored at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.
Tissue specificity	Expressed in placenta and several fetal tissues.
Celluar localization	Endosome;Plasma Membrane;
UniPort	P55073