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Rabbit Anti-CKII Alpha Polyclonal Antibody#abs137044

Please be informed that the provided price is only for your reference. For detailed pricing, we kindly suggest reaching out to our seller, Vecent. Western blot analysis CKII alpha using HepG2 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in...

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Description

Catalog-specification	Delivery time	USD price
abs137044-100ug	1-2 Weeks	301.0
abs137044-50ug	1-2 Weeks	201.0

Please be informed that the provided price is only for your reference. For detailed pricing, we kindly suggest reaching out to our seller, Vecent.

Overview
catalog	abs137044
Other names	The protein known as casein kinase 2 alpha 1 polypeptide is also referred to as casein kinase II alpha 1, or simply CK II alpha. This polypeptide is a subunit of the protein kinase known as casein kinase II, and serves as the catalytic subunit of this enzyme. In humans, this subunit is encoded by the CSNK2A1 gene. Casein kinase II plays an important role in regulating a wide range of cellular processes, including cell division, gene expression, and apoptosis. Dysregulation of casein kinase II has been implicated in several diseases, including cancer and neurodegenerative disorders.
Source	Rabbit
Specificity	CKII alpha Antibody is capable of detecting the levels of total CKII alpha that occur naturally within an organism. This antibody specifically targets and binds to CKII alpha, allowing for the measurement and quantification of its presence in an accurate and reliable manner.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Bovine;Sheep;
Antigen	CKII alpha
Application	For Western blotting, dilute the antibody at a ratio of 1:1000-3000. When performing immunohistochemistry, a dilution of 1:200 would be appropriate. For immunofluorescence/immunocytochemistry, dilute the antibody at a ratio of 1:100-1:500 depending on the application. For ELISA using a peptide substrate, a dilution of 1:20000-1:40000 should be used. It's important to keep in mind the appropriate dilution when using this antibody in different applications.
Immunogen	A synthetic peptide originating from human CKII alpha has been created. It closely resembles the original protein and was synthesized through careful manipulation of chemical compounds. This achievement represents a significant step forward in our understanding of protein structure and function, and may have important implications for the development of new therapies and treatments. The peptide's highly similar composition to natural CKII alpha suggests that it may be able to mimic the protein's biological activity, paving the way for further research in this area.
MW	45 kDa
Properties
Concentration	1mg/ml
purification	The purification process for the antiserum involved the use of SulfoLink™ Coupling Resin for peptide affinity chromatography. This allowed for highly specific binding of the target peptides, resulting in a purified product of high quality.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20°C for a duration of one year. It is important to avoid repeated freeze and thaw cycles to prevent any potential damage to the substance. Taking these precautions will ensure that the product remains usable for its intended purpose and maintains its effectiveness.
Storage buffer	The Rabbit IgG can be stored at -20 °C in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. This solution is stable for 12 months from the date of receipt, making it an ideal option for long-term storage. Keep in mind that the solution should be handled with care and caution to ensure the integrity and quality of the Rabbit IgG is maintained.
Target
Background	Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at 'Ser-565' and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at 'Ser-90' which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry (PubMed:11239457, PubMed:11704824, PubMed:16193064, PubMed:19188443, PubMed:20625391, PubMed:22406621). Phosphorylates CCAR2 at 'Thr-454' in gastric carcinoma tissue (PubMed:24962073).
Tissue specificity	Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level).
Posttranslational modification	Phosphorylated at Thr-344, Thr-360, Ser-362 and Ser-370 by CDK1 in prophase and metaphase and dephosphorylated during anaphase. Phosphorylation does not directly affect casein kinase 2 activity, but may contribute to its regulation by forming binding sites for interacting proteins and/or targeting it to different compartments.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	P68400