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Rabbit Anti-C/EBP-beta Polyclonal Antibody#abs131744

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Description

Catalog-specification	Delivery time	USD price
abs131744-100ug	1-2 Weeks	301.0
abs131744-50ug	1-2 Weeks	201.0

Overview
catalog	abs131744
Description	This intronless gene encodes a bZIP transcription factor that is responsible for producing a specific protein. The protein has the ability to bind to certain DNA regulatory regions either as a homodimer or as a heterodimer with other related proteins such as CEBP-alpha, CEBP-delta, and CEBP-gamma. This transcription factor plays a crucial role in regulating gene expression and controlling various cellular processes. Its ability to form different types of dimers enhances its functional versatility and enables it to interact with different DNA regions, thereby modulating gene activity.
Other names	Below is a rearranged version of the content: CCAAT Enhancer Binding Protein beta (CEBPβ), also known as AGP/EBP or C/EBP related protein 2, is a transcription factor that plays an important role in various biological processes. This protein, encoded by the CEBPB gene, is also referred to as C/EBP beta or IL6DBP. It is involved in regulating the expression of genes and is known to bind to the enhancer regions of DNA. CEBPβ, or LAP, is a liver-enriched transcriptional activator that can interact with other proteins to regulate the transcription of specific genes. It is also known as NF IL6 or NF-IL6, which indicates its involvement in the NF-IL6 pathway. Another name for this protein is TCF-5, and it is sometimes referred to as Silencer factor B or SF B. This protein has been found to have a binding affinity for Interleukin 6 (IL-6) and is known as IL6DBP or IL 6 dependent binding protein. This interaction suggests its potential role in mediating the effects of IL-6 signaling. Overall, CEBPβ is a versatile transcription factor with several names, reflecting its involvement in different cellular pathways and its ability to regulate gene expression in various tissues.
Source	Rabbit
Specificity	The detection of total C/EBP-beta is achieved by the C/EBP-beta Antibody, which specifically targets the endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog;Bovine;
Antigen	C/EBP-β
Application	The recommended dilutions for Western blotting range from 1:500 to 1:2000. For immunohistochemistry, the suggested dilutions are between 1:50 and 1:200. When it comes to immunofluorescence/immunocytochemistry, the appropriate dilutions to use are 1:100 to 1:500. Lastly, for ELISA using peptides, an ideal dilution would be within the range of 1:20000 to 1:40000. It is important to follow these dilution guidelines to ensure accurate and reliable results in your experiments.
Immunogen	A peptide that has been synthesized from the human C/EBP-beta molecule is available. To create content that is highly similar to this statement, one could rephrase it as follows: It is possible to obtain a synthesized peptide by utilizing the human C/EBP-beta molecule. This peptide may have a high degree of similarity to the original molecule from which it was derived.
MW	36kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography for purification. A highly refined antiserum was achieved through this process.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:9374525, PubMed:12048245, PubMed:18647749). Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:20829347). Essential for female reproduction because of a critical role in ovarian follicle development (By similarity). Restricts osteoclastogenesis (By similarity).
Tissue specificity	Expressed at low levels in the lung, kidney and spleen.
Posttranslational modification	Methylated. Methylation at Arg-3 by CARM1 and at Lys-43 by EHMT2 inhibit transactivation activity. Methylation is probably inhibited by phosphorylation at Thr-235.Sumoylated by polymeric chains of SUMO2 or SUMO3 (PubMed:12810706). Sumoylation at Lys-174 is required for inhibition of T-cells proliferation. In adipocytes, sumoylation at Lys-174 by PIAS1 leads to ubiquitination and subsequent proteasomal degradation. Desumoylated by SENP2, which abolishes ubiquitination and stabilizes protein levels (By similarity).Ubiquitinated, leading to proteasomal degradation.Phosphorylated at Thr-235 by MAPK and CDK2, serves to prime phosphorylation at Thr-226 and Ser-231 by GSK3B and acquire DNA-binding as well as transactivation activities, required to induce adipogenesis. MAPK and CDK2 act sequentially to maintain Thr-235 in the primed phosphorylated state during mitotical cloning expansion and thereby progression of terminal differentiation. Phosphorylation at Thr-266 enhances transactivation activity. Phosphorylation at Ser-325 in response to calcium increases transactivation activity. Phosphorylated at Thr-235 by RPS6KA1 (PubMed:11684016).O-glycosylated, glycosylation at Ser-227 and Ser-228 prevents phosphorylation on Thr-235, Ser-231 and Thr-226 and DNA binding activity which delays the adipocyte differentiation program.Acetylated. Acetylation at Lys-43 is an important and dynamic regulatory event that contributes to its ability to transactivate target genes, including those associated with adipogenesis and adipocyte function. Deacetylation by HDAC1 represses its transactivation activity. Acetylated by KAT2A and KAT2B within a cluster of lysine residues between amino acids 129-133, this acetylation is strongly induced by glucocorticoid treatment and enhances transactivation activity.
Celluar localization	Nucleus;
UniPort	P17676