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Rabbit Anti-ARF1 Polyclonal Antibody#abs137127

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Description

Catalog-specification	Delivery time	USD price
abs137127-100ug	1-2 Weeks	301.0
abs137127-50ug	1-2 Weeks	201.0

Overview
catalog	abs137127
Other names	ARF1, also known as ADP Ribosylation Factor 1, is a protein that plays a crucial role in intracellular vesicular trafficking and membrane remodeling. ARF1 is a member of the ADP-ribosylation factor family and is involved in the regulation of various cellular processes. The protein ARF1 is encoded by the ARF1 gene in humans. It is known to be widely expressed in various tissues and cell types. ARF1 functions as a regulator of vesicular transport through its ability to cycle between an inactive GDP-bound form and an active GTP-bound form. ARF1 acts as a molecular switch, controlling membrane trafficking pathways by interacting with specific effector proteins. When in the GTP-bound form, ARF1 recruits various effectors to the Golgi complex, endosomes, and plasma membrane, where it coordinates membrane budding, vesicle formation, and fusion events. ARF1 is involved in the regulation of many cellular processes, including protein sorting, membrane trafficking, and organelle biogenesis. It plays a crucial role in the formation of transport vesicles from the Golgi complex and is essential for maintaining the integrity and functionality of the secretory pathway. In addition to its role in intracellular trafficking, ARF1 has also been implicated in the regulation of cytoskeletal dynamics, cell adhesion, and signal transduction. It interacts with several proteins involved in these processes, including cytoskeletal components, membrane fusion machinery, and signal transduction molecules. Overall, ARF1 is a versatile and important protein involved in a wide range of cellular processes. Its dynamic regulation and interaction with various effectors make it a key player in maintaining cellular homeostasis and proper vesicular transport.
Source	Rabbit
Specificity	The ARF1 Antibody is capable of detecting total ARF1 levels that naturally occur within the body.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Zebrafish;Pig;Sheep;Rabbit;Bovine;Chicken
Antigen	ARF1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human ARF1.
MW	20 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we were able to purify the antiserum through peptide affinity chromatography. The resulting product is highly targeted and effective in treating the specific antigen it was designed for.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may affect its properties. Store it consistently at the recommended temperature to ensure its stability and effectiveness over time.
Storage buffer	The provided content contains information about a storage solution for Rabbit IgG. Here is a rearranged version: Storage Solution for Rabbit IgG: To store Rabbit IgG effectively, it is recommended to use a phosphate-buffered saline solution with a pH value of 7.4. This solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. Maintaining a temperature of -20 °C is crucial for optimal storage. It is important to note that this storage solution remains stable for a period of 12 months starting from the date of receipt.
Target
Background	GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Posttranslational modification	Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3.
Celluar localization	Cytosol;Endosome;Extracellular region or secreted;Golgi apparatus;Peroxisome;Plasma Membrane;
UniPort	P84077