Phospho-MLK3 (Thr277+Ser281) Rabbit Polyclonal Antibody#abs139908
Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. Western blot analysis MLK3 (Phospho-Thr277+Ser281) using K562 whole cell lysates This product is for research use only, not for use in diagnostic...
Description
| Catalog-specification | Delivery time | USD price |
abs139908-100ug | 1-2 Weeks | 301.0 |
abs139908-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent.
| Overview | |
catalog | abs139908 |
| Other names | Protein tyrosine kinase PTK1, also known as MEKK11 or Mitogen-activated protein kinase kinase kinase 11, plays a crucial role in cellular signaling pathways. It is encoded by the gene 2610017K16Rik in humans. Another name for this protein is M3K11_HUMAN or Map3k11. Additionally, it is referred to as Mixed lineage kinase 3 (Mlk-3), Mixed lineage protein kinase 3 (Mlk3), or Src-homology 3 domain-containing proline-rich kinase (SPRK). MGC17114 is another identifier associated with this protein. RHOE, a related gene, is also linked to PTK1. The function of PTK1 is to initiate a cascade of signaling events involved in various biological processes. In summary, Protein tyrosine kinase PTK1, known by multiple names such as MEKK11, M3K11_HUMAN, or Map3k11, is an important player in cellular signaling pathways and holds significance in scientific research. |
| Source | Rabbit |
| Specificity | The MLK3 (Phospho-Thr277+Ser281) Antibody is capable of detecting the presence of MLK3 protein only when it is phosphorylated at specific sites, namely Thr277 and Ser281. This antibody specifically recognizes and binds to the phosphorylated form of MLK3, enabling researchers to study its signaling pathways and cellular functions. The detection of endogenous levels of MLK3 can provide valuable insights into its role in various biological processes. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Pig;Rabbit;Sheep;Dog;Bovine;Horse; |
| Antigen | MLK3 |
| Application | WB 1:1000-3000 is the recommended dilution range for Western Blot analysis, indicating the optimal concentration of the primary antibody to be used. For Immunohistochemistry (IHC), the recommended dilution range is 1:50-1:200. This dilution range helps ensure accurate and specific staining of the target protein in tissue sections. When using ELISA with a peptide antigen, the recommended dilution range for the primary antibody is 1:20000-1:40000. This dilution range allows for optimal detection of the target antigen in the ELISA assay. It is important to note that these dilution ranges may vary depending on the specific experimental conditions and the target protein being analyzed. Therefore, it is recommended to optimize the dilution for each antibody and experimental setup to obtain reliable and reproducible results. |
| Immunogen | A peptide has been synthesized that is derived from the human MLK3 protein and contains phosphorylated threonine at position 277 and serine at position 281. In order to create similar content, one might rearrange these elements to state that a synthesized peptide has been derived from human MLK3 and is phosphorylated at both threonine 277 and serine 281. Both formulations convey the same basic information about the composition of the synthesized peptide. |
| MW | 92KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum was used to extract the antibody, which was further purified through the use of affinity purification via sequential chromatography. This process involved the use of both phospho- and non-phospho-peptide affinity columns to ensure the high quality of the final product. The resulting antibody is highly effective and can be used for a variety of applications. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | One should ensure to store the product at a temperature of -20 °C for a duration of one year. It is recommended to prevent exposure to repeated cycles of freezing and thawing. |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle. |
| Tissue specificity | Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain. |
| Posttranslational modification | Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42. |
| Celluar localization | Cytoskeleton; |
| UniPort | Q16584 |
Western blot analysis MLK3 (Phospho-Thr277+Ser281) using K562 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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