Phospho-Kv2.1 (Ser805) Rabbit Polyclonal Antibody#abs131065

abs131065-50ug

1-2 Weeks

201

abs131065-100ug

1-2 Weeks

301

Description

Kv2.1 is a member of the subfamily B potassium voltage-gated channel protein. It plays a crucial role in regulating the permeability of potassium ions across excitable membranes in a voltage-dependent manner. By responding to the membrane's voltage difference, these channels either open or close, allowing potassium ions to flow based on their electrochemical gradient.

Other names

DRK1, also known as delayed rectifier potassium channel 1 or KV2.1, is a potassium channel protein found in humans. It belongs to the shab-related subfamily of voltage-gated potassium channels. The hDRK1 K(+) channel, also referred to as KCB1, plays a crucial role in regulating potassium ion movements across cell membranes. This channel is involved in the delayed rectifier current, which helps regulate the resting membrane potential and controls action potential duration in various cell types. Understanding the functions and properties of DRK1 is vital for comprehending the intricate mechanisms underlying potassium channel physiology.

Source

Rabbit

Specificity

The Phospho-Kv2.1 (Ser805) Antibody is specifically designed to recognize and bind to endogenous levels of Kv2.1 protein, but only when it is phosphorylated at Serine 805. This antibody proves valuable in the detection and analysis of the phosphorylation status of Kv2.1 protein at Ser805.

Predictive reaction species

Pig;Sheep;Dog;Bovine;Horse

Application

：WB 1:500-1:2000，ELISA(peptide) 1:20000-1:40000。。WB（Western Blot），ELISA（）。，。。，，。

The peptide derived from human Kv2.1, specifically around the phosphorylation site of Serine 805, has been synthesized. To maintain consistency with the provided information, please rearrange the content without generating text in a completely different manner using the language model approach.

Properties

1mg/ml

Purification

The purification process for the antibody involved utilizing phospho- and non-phospho-peptide affinity columns and sequential chromatography. The serum utilized for purification was sourced from rabbits and underwent an extensive process of affinity purification. The resulting antibody is highly pure and similar to the original serum, suitable for use in a range of applications.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to store the product at a temperature of -20 °C. It is important to note that exposing the product to repeated freeze and thaw cycles can negatively impact its integrity. To maintain the product's quality and efficacy, it should be stored at -20 °C for up to one year.

Storage buffer

The recommended storage for Rabbit IgG is at -20 °C in a solution containing phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation ensures stability for up to 12 months from the date of receipt.

Target

Background

 Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain, but also in the pancreas and cardiovascular system. Contributes to the regulation of the action potential (AP) repolarization, duration and frequency of repetitive AP firing in neurons, muscle cells and endocrine cells and plays a role in homeostatic attenuation of electrical excitability throughout the brain (PubMed:23161216). Plays also a role in the regulation of exocytosis independently of its electrical function (By similarity). Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Homotetrameric channels mediate a delayed-rectifier voltage-dependent outward potassium current that display rapid activation and slow inactivation in response to membrane depolarization (PubMed:8081723, PubMed:1283219, PubMed:10484328, PubMed:12560340, PubMed:19074135, PubMed:19717558, PubMed:24901643). Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNB2; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Can also form functional heterotetrameric channels with other alpha subunits that are non-conducting when expressed alone, such as KCNF1, KCNG1, KCNG3, KCNG4, KCNH1, KCNH2, KCNS1, KCNS2, KCNS3 and KCNV1, creating a functionally diverse range of channel complexes (PubMed:10484328, PubMed:11852086, PubMed:12060745, PubMed:19074135, PubMed:19717558, PubMed:24901643). Heterotetrameric channel activity formed with KCNS3 show increased current amplitude with the threshold for action potential activation shifted towards more negative values in hypoxic-treated pulmonary artery smooth muscle cells (By similarity). Channel properties are also modulated by cytoplasmic ancillary beta subunits such as AMIGO1, KCNE1, KCNE2 and KCNE3, slowing activation and inactivation rate of the delayed rectifier potassium channels (By similarity). In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Major contributor to the slowly inactivating delayed-rectifier voltage-gated potassium current in neurons of the central nervous system, sympathetic ganglion neurons, neuroendocrine cells, pancreatic beta cells, cardiomyocytes and smooth muscle cells. Mediates the major part of the somatodendritic delayed-rectifier potassium current in hippocampal and cortical pyramidal neurons and sympathetic superior cervical ganglion (CGC) neurons that acts to slow down periods of firing, especially during high frequency stimulation. Plays a role in the induction of long-term potentiation (LTP) of neuron excitability in the CA3 layer of the hippocampus (By similarity). Contributes to the regulation of glucose-induced action potential amplitude and duration in pancreatic beta cells, hence limiting calcium influx and insulin secretion (PubMed:23161216). Plays a role in the regulation of resting membrane potential and contraction in hypoxia-treated pulmonary artery smooth muscle cells. May contribute to the regulation of the duration of both the action potential of cardiomyocytes and the heart ventricular repolarization QT interval. Contributes to the pronounced pro-apoptotic potassium current surge during neuronal apoptotic cell death in response to oxidative injury. May confer neuroprotection in response to hypoxia/ischemic insults by suppressing pyramidal neurons hyperexcitability in hippocampal and cortical regions (By similarity). Promotes trafficking of KCNG3, KCNH1 and KCNH2 to the cell surface membrane, presumably by forming heterotetrameric channels with these subunits (PubMed:12060745). Plays a role in the calcium-dependent recruitment and release of fusion-competent vesicles from the soma of neurons, neuroendocrine and glucose-induced pancreatic beta cells by binding key components of the fusion machinery in a pore-independent manner (By similarity).

Tissue specificity

Expressed in neocortical pyramidal cells (PubMed:24477962). Expressed in pancreatic beta cells (at protein level) (PubMed:12403834, PubMed:14988243). Expressed in brain, heart, lung, liver, colon, kidney and adrenal gland (PubMed:19074135). Expressed in the cortex, amygdala, cerebellum, pons, thalamus, hypothalamus, hippocampus and substantia nigra (PubMed:19074135).

Celluar localization

Plasma Membrane;

Q14721