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Phospho-JIP1 (Thr103) Rabbit Polyclonal Antibody#abs138260

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Description

Catalog-specification	Delivery time	USD price
abs138260-100ug	1-2 Weeks	301.0
abs138260-50ug	1-2 Weeks	201.0

Overview
catalog	abs138260
Other names	JNK-interacting protein 1, also known as C-jun-amino-terminal kinase-interacting protein 1 or IB1, is a crucial scaffold protein that interacts with the JNK MAP kinase. It is also referred to as Islet Brain 1 or JIP-1. This protein, encoded by the MAPK8IP1 gene, plays a significant role in various cellular processes. The MAPK8IP1 gene produces a protein known as mitogen-activated protein kinase 8-interacting protein 1. PRKM8IP is another name for this protein. It acts as a mediator between JNK and its downstream targets. By facilitating the assembly of JNK signaling complexes, JIP-1 aids in efficient JNK signaling. JIP-1 is involved in numerous cellular functions, including cell survival, gene expression, neuronal development, and synaptic plasticity. It is highly expressed in the brain, particularly in neuronal tissues. By acting as a scaffolding protein, JIP-1 brings together JNK and its substrates, enabling specific signaling pathways. Understanding the role of JIP-1 is essential for elucidating the complexities of JNK signaling. Disruptions in JIP-1 function have been implicated in various diseases and conditions, including neurodegenerative disorders and cancer. Thus, further research on JIP-1 may provide valuable insights into potential therapeutic targets and interventions.
Source	Rabbit
Specificity	The Phospho-JIP1 (Thr103) Antibody is specifically designed to recognize JIP1 only in its phosphorylated form at Thr103. It is highly effective in detecting the endogenous levels of this protein, which is essential for many cellular processes. With the help of this antibody, researchers can gain valuable insights into the role played by JIP1 in various physiological and pathological conditions. The antibody can be used in a variety of experiments, including western blotting, immunoprecipitation, and immunofluorescence. Overall, the Phospho-JIP1 (Thr103) Antibody is a powerful tool for elucidating the complex functions of JIP1 in biological systems.
Species Reactivity	Human;Mouse;Rat
Antigen	JIP1
Application	Here are some alternative phrasings based on the original text information: - For Western blotting, dilute the primary antibody at a range of 1:1000 to 1:3000. When using immunofluorescence/immunocytochemistry, employ a dilution of 1:100 to 1:500. For ELISA with a peptide antigen, use a dilution series of 1:20000 to 1:40000. - The recommended dilutions for primary antibody in Western blotting is between 1:1000 to 1:3000. For immunofluorescence/immunocytochemistry, dilute the antibody at 1:100 to 1:500. When performing ELISA with a peptide antigen, use a dilution series ranging from 1:20000 to 1:40000. - Depending on the application, dilute the primary antibody for Western blotting in a range of 1:1000 to 1:3000. For immunofluorescence/immunocytochemistry, use a dilution range of 1:100 to 1:500. In ELISA experiments using a peptide antigen, dilute the antibody over a series of 1:20000 to 1:40000. - Use a dilution range of 1:1000 to 1:3000 for primary antibody in Western blotting experiments. For immunofluorescence/immunocytochemistry, dilute the antibody at 1:100 to 1:500. In ELISA tests using a peptide antigen, dilute the antibody with a dilution series spanning from 1:20000 to 1:40000.
Immunogen	From human Phospho-JIP1 (Thr103), a peptide has been synthesized. To generate a highly similar content, the original text information must be rearranged. However, please note that the conversation should not be conducted based on the method of generating content using ChapGPT, but rather by speaking in a completely different manner using a language model.
MW	77 kDa
Properties
Concentration	1mg/ml
purification	The purified rabbit serum produces the antibody, which is subject to sequential chromatography on phospho- and non-phospho-peptide affinity columns for affinity purification. With this process, a highly specific and purified antibody is obtained for further use.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve quality, it is recommended to store at a temperature of -20°C for up to a year. It is important to avoid repeatedly subjecting the product to freeze-and-thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response.
Tissue specificity	Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis.
Posttranslational modification	Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases.Ubiquitinated. Two preliminary events are required to prime for ubiquitination; phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.
Celluar localization	Cytosol;Endoplasmic reticulum;Mitochondrion;Nucleus;
UniPort	Q9UQF2