Tubulin Beta Antibody #abs131994

abs131994-50ug

In Stock

201

abs131994-100ug

In Stock

301

Description

TUBB3, also known as Tubulin, is the primary building block of microtubules. It has a strong affinity for GTP and can bind two molecules of it. The beta chain of TUBB3 has an exchangeable GTP site, while the alpha-chain has a non-exchangeable GTP site. The proper functioning of TUBB3 is crucial for the guidance and maintenance of axons.

Source

Rabbit

Specificity

The Tubulin beta Antibody is designed to specifically recognize and bind to endogenous levels of total Tubulin beta. It is a reliable tool for the detection and analysis of Tubulin beta protein in various applications. By efficiently detecting the target protein, this antibody enables researchers to study its functions and interactions in different biological processes.

Species Reactivity

Human;Mouse;Rat

Application

For Western blotting, dilution ranges of 1:1000 to 1:5000 are recommended, while for immunohistochemistry, dilution ranges of 1:50 to 1:200 are optimal. When using the protein for immunofluorescence or immunocytochemistry, a dilution of 1:100 to 1:500 is suggested. When conducting an ELISA with the peptide, a dilution range of 1:20,000 to 1:40,000 is recommended for optimal results. To ensure that accurate and reliable results are obtained, it is important to carefully adhere to the recommended dilution ranges for each application.

Immunogen

A newly developed peptide has been synthesized from human Tubulin beta. This peptide is very similar to the original sequence and is based on the information contained in the primary amino acid structure of Tubulin beta. The development of this peptide has potential applications in various fields such as medicine and biotechnology. With further research and experimentation, this peptide may have the potential to become a valuable therapeutic agent for various diseases.

Properties

Concentration

1mg/ml

Purification

We utilized SulfoLink™ Coupling Resin for peptide affinity chromatography in order to purify the antiserum. Please rearrange the given content and ensure that the resulting content is highly similar, based on the original text information.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality and stability of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze and thaw cycles, as this can lead to changes in its composition and structure. By following these storage guidelines, you can ensure that the product remains effective and reliable over time.

Storage buffer

This product is Rabbit IgG suspended in a solution of phosphate buffered saline at pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended that the product be stored at a temperature of -20°C and is stable up to 12 months from receipt. Please be sure to handle the product with care, following the proper storage procedures to ensure its efficacy.

Target

Background

Microtubules are largely composed of tubulin, which interacts with GTP in a specific manner. Specifically, one molecule of GTP binds to the beta chain at a site that can be replaced, while another binds to the alpha chain at a non-replaceable site. TUBB3, on the other hand, is critically involved in maintaining proper axon guidance and ensuring its maintenance.

Tissue specificity

The expression of the gene is mainly limited to the central and peripheral nervous systems. There is a significant rise in expression in the majority of malignant tissues. Please create a closely related statement by reorganizing the provided information, while keeping the content consistent with the original text. Please do not engage in a conversation like the one generated by ChatGPT, but rather deliver a speech in a completely different manner using the language model to generate the text.

Posttranslational modification

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Celluar localization

Cytoskeleton;Extracellular region or secreted;Nucleus;

UniPort

Q13509