Rabbit Anti-tubulin Beta Polyclonal Antibody #abs125209

abs125209-50ul

In Stock

201

abs125209-100ul

In Stock

301

Source

Rabbit

Specificity

Beta tubulin

Species Reactivity

Human;Mouse;Rat;Rabbit

Application

The dilution ranges for WB are 1:2000 to 10000, for ELISA they are 1:500 to 1000, and for IHC-P and IHC-F they are 1:400 to 800. IF has a dilution range of 1:100 to 500, but paraffin sections require antigen repair. It is important to note that these dilution ranges may vary depending on the specific experiment and reagents used, so it is recommended to optimize dilutions for each individual experiment.

Immunogen

This synthetic peptide is derived from the human tubulin Beta sequence, specifically the region between amino acids 61-160 out of a total of 444. It has been conjugated with KLH for use in various research applications. To create a highly similar content, one could rearrange the above statement by saying that the KLH-conjugated synthetic peptide is derived from human tubulin Beta and encompasses amino acids 61-160 out of a total of 444. This peptide has been developed for use in research and has several applications.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To preserve the integrity of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. Care should be taken to avoid subjecting the product to multiple freeze/thaw cycles, as this may compromise its quality.

Storage buffer

，：
1% BSA、0.03% Proclin30050%pH7.40.01M TBS。

Research area

Immunology neurobiology cytoskeleton

Target

Background

Alpha- and beta-tubulin, two soluble proteins with a molecular weight of approximately 55,000 kilodaltons each, make up the key components of microtubules. These specialized structures can be found in various parts of the cell, including the mitotic apparatus, cilia, flagella, and cytoskeleton. Microtubules play crucial roles in cell division, cellular movement, and maintaining cell shape and stability. Their dynamic nature and ability to assemble and disassemble are essential for numerous cellular processes.
When it comes to Western Blotting, antibodies that target beta Tubulin are commonly employed as loading controls. However, it is important to keep in mind that the levels of beta Tubulin within certain cells may not be consistent. For instance, adipose tissue exhibits very low expression of tubulin (as pointed out in Spiegelman and Farmer's 1982 publication in Cell), and thus the use of beta Tubulin as a loading control for these types of tissues would not be appropriate.

Celluar localization

Cytoplasm

UniPort

P07437