Recombinant Mouse LIGHT/TNFSF14 #abs01002

abs01002-5ug

1-2 Weeks

138

abs01002-20ug

1-2 Weeks

344

abs01002-1mg

1-2 Weeks

3891

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH7.4 was filtered through a 0.2 μm filter and then lyophilized to yield a dry powder. This process resulted in a highly stable product that is easy to store and transport. The resulting powder can be easily reconstituted with water or other solvents to yield a solution that is highly similar to the original concentrated solution. This product is ideal for a wide range of applications in the pharmaceutical, biotechnology, and life science industries. Its stability, purity, and consistency make it a valuable tool for research and development, as well as for production and manufacturing.

Properties

aa_sequence

RGTYHDGAL LGGVVHLEAG DGGKGSWEKL PAAHLTGANA SSRVWWDSSF YSKVQLSGVG SRRSPCGRAN IQDQRSHQAN WETRLGLAFL VLVRPRDGTR SLIGIGGPLL VTMEPGYYYV RYPKELELLV EEVVVRVPGN CPQGLANGLP ITHGLYKRTS SYFGAFMV。
Here's a rearrangement of the above content:
The sequence DGGKGSWEKL, IQDQRSHQAN, PAAHLTGANA, SLIGIGGPLL, WETRLGLAFL, RGLTYHDGAL, VTMEPGYYYV, YSKVQLSGVG, CPQGLANGLP, ITHGLYKRTS, RYPKELELLV, SRRSPCGRAN, SSRVWWDSSF, LGGVVHLEAG, EEVVVRVPGN, RLVRPRDGTR, and SYFGAFMV can be rearranged to form sentences.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The determined amount of rMuLIGHT/TNFSF14 using the LAL method is less than 1 EU/μg. Let's present the information in a different way: The LAL method indicated that the concentration of rMuLIGHT/TNFSF14 is below 1 EU/μg.

Activity

The standard and the fully biologically active versions have no significant differences. The ED is comparable to that of standard.₅₀The specific activity of the determined cytotoxicity assay using human HT-29 cells is greater than 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and Recombinant HumanIFN-γ, with a corresponding concentration of less than 2 μg/ml. Let's rearrange the content to create a highly similar version based on the original information:
The specific activity of the cytotoxicity assay using human HT-29 cells was evaluated, resulting in a concentration of less than 2 μg/ml. This corresponds to a remarkable specific activity of over 500 IU/mg when combined with murine anti-polyHistidine monoclonal antibody and Recombinant HumanIFN-γ.

Reconstitution

To ensure proper mixing of the contents, it is advised to briefly centrifuge the vial before opening. Afterwards, reconstitute the contents in 100 mM HAc to achieve a concentration of 0.1-1.0 mg/ml. To maintain efficacy, divide the stock solutions into smaller working aliquots and store them at temperatures below or equal to -20 °C. When making further dilutions, use appropriate buffered solutions for optimum results. Remember to avoid using the method of dialogue generation from ChapGPT and instead generate content that is completely distinct using the language model.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C but for long term storage, desiccated storage at -20 °C is preferred. Once reconstituted, the preparation can last up to one week when stored at 2-8°C. To maintain its stability, it is advisable to divide the reconstituted preparation into working aliquots and store at -20 to -70°C. It is best to refrain from repeating freeze/thaw cycles, as this can lead to instability.