Recombinant PreScission Protease #abs01243

abs01243-100IU

1-2 Weeks

275

abs01243-250IU

1-2 Weeks

523

abs01243-5000IU

1-2 Weeks

7318

Description

The PreScission Protease is a fusion protein consisting of human rhinovirus (HRV) type 14 3C protease and glutathione S-transferase (GST). This protease has a specific affinity towards certain sequences that include the Leu-Phe-Gln/Gly-Pro core amino acid sequence, cleaving at the glycine residue after the glutamine. The recognition and cleavage of substrates not only rely on primary structural signals but also on the secondary and tertiary structures of the fusion protein.

A unit can be defined as the minimum quantity of enzyme required to hydrolyze 100 μg of fusion protein within a time span of 16 hours, achieving 90% completion. These conditions are maintained at a temperature of 5 °C in a buffer solution that contains 50 mM Tris-HCl (pH 7.0), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. It is important to note that the generated content should not be based on the original text information but should be different in nature.

Other names

3C protease, Picornain 3C, PSP

Species

Viral

Source

Escherichia coli.

Properties

Reconstitution

The cleavage buffer used in this experiment contains 50 mM Tris-HCl at pH 7.0 when measured at 25°C, 150 mM NaCl, 1 mM EDTA and 1 mM dithiothreitol. To ensure optimal performance, it is recommended to chill the buffer to 5°C prior to use.

Stability & Storage

For long term storage, it is recommended to keep the substance in small portions and store them at a temperature of -20°C. It is important to preserve the original text information while generating highly similar content by rearranging the given sentences. One can use language model techniques to come up with different and unique ways to convey the same information.

Warnings

For optimal cleavage of fusion proteins, it is recommended to monitor the reaction by taking samples at different time points and running SDS-PAGE analysis. The conditions necessary for complete digestion may vary depending on the fusion partner; therefore, pilot experiments should be performed to determine optimal conditions. Triton X-100, Tween 20, or Nonidet P40 can be added to the reaction at a concentration of 0.01% to improve digestion, with concentrations up to 1% not affecting PreScission Protease activity. It is crucial to adjust the amount of PreScission Protease, temperature, and incubation time to ensure high yield and purity of the target protein. By analyzing the extent of the reaction, you can determine whether further optimization of conditions is needed to achieve complete digestion of the fusion protein.

References

References

1. Werner G, Rosenwirth B, Bauer E, et al. 1986. J Virol, 57: 1084-93.

2. Libby RT, Cosman D, Cooney MK, et al. 1988. Biochemistry, 27: 6262-8.

3. Aschauer B, Werner G, McCray J, et al. 1991. Virology, 184: 587-94.

4. Leong LE, Walker PA, Porter AG. 1993. J Biol Chem, 268: 25735-9.