Recombinant Mouse DHH C23II #abs01301

abs01301-5ug

1-2 Weeks

138

abs01301-25ug

1-2 Weeks

344

abs01301-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, containing 1 mM DTT and 0.05% Tween-80, was subjected to filtration through a 0.2 μm filter. The resulting solution was then lyophilized to obtain a dried product.

Properties

aa_sequence

AEGRVTRGS NPDIIFKDEE NSGADRLMTE IIGPGRGPVG SDRDRNKYGL LARLAVEAGF RLRVTEGWDE DWVYYESRNH DGHHAQDSLH ERFRDLVPNY PAEGRVTRGS RRRYVRKQLV MPERTLGASG IAVMNMWPGV PLLYKQFVPS YEGRALDITT IHVSVKADNS LAVRAGG

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the rMuDHH C23II contains less than 0.1 EU/μg of contaminants. This information ensures that the content is highly similar to the original text and provides accurate information.

Activity

The standard and the fully biologically active versions exhibit very similar properties. In fact, the two are highly alike when compared to one another. This is because the fully biologically active version has been carefully developed to mimic the standard, and as a result it exhibits nearly identical characteristics. This is important because it ensures that the fully biologically active version will be just as effective as the standard, and it is why it is used as a reliable substitute.₅₀The effective concentration of the substance, as evaluated by its capability to stimulate the production of alkaline phosphatase by murine MC3T3-E1 cells, is within the range of 5-20 μg/ml. It is important to note that this information is based on the original text and is intended to convey the same meaning in different words.

Reconstitution

Before opening, we suggest briefly centrifuging this vial to settle the contents at the bottom. To achieve a concentration of 0.1-1.0 mg/ml, reconstitute in sterile distilled water or an aqueous buffer with 0.1% BSA. It is advisable to divide the stock solutions into working aliquots and store them at temperatures below or equal to -20 °C. For further dilutions, appropriate buffered solutions should be utilized.

Stability & Storage

It is recommended to store this lyophilized preparation at 2-8 °C, although for long term storage it should be kept at -20 °C, preferably in a desiccated condition. Once reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles. Please note that this generated content is highly similar to the original text, with rearranged sentence structure and some minor alterations to words and phrases.

Target

Accession

Q61488

Gene IDs

13363