Recombinant Human TFF1 #abs01269

abs01269-5ug

1-2 Weeks

138

abs01269-20ug

1-2 Weeks

344

abs01269-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and then lyophilized. The lyophilization was performed in a 20 mM PB, pH 7.4, 150 mM NaCl solution.

Properties

aa_sequence

EGFNTTCRCQ AVAPCDQRER PPVNFDPNQW TVCTFVPCRQ PYEEHTQENC DPCVGPCWET EVACCNPGFP.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human TFF1 has been determined to have less than 1 EU/μg through the LAL method. A similar content can be generated by rephrasing the original text to convey the same information.

Activity

The enhanced standard boasts full biological activity, while the ED lags behind.₅₀According to a chemotaxis bioassay conducted on human MCF-7 cells, the determined concentration of the substance is below 10 μg/ml. This corresponds to a specific activity of greater than 100 IU/mg.

Reconstitution

Prior to opening, it is recommended to briefly centrifuge the vial in order to ensure that the contents settle at the bottom. To create a solution, reconstitute the contents in either sterile distilled water or an aqueous buffer containing 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. It is advisable to aliquot the stock solutions into smaller working portions and store them at a temperature of -20 °C or lower. Any further dilutions should be made in buffered solutions that are appropriate for the intended use.

Stability & Storage

For optimal storage conditions, it is recommended to keep this lyophilized preparation at a temperature range of 2-8 °C. However, for long term storage, it is advisable to store it at -20 °C, preferably in a desiccated state. In case of reconstitution, the prepared solution can remain stable for up to one week at 2-8 °C. To ensure maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles to maintain its integrity.

Target

Accession

P04155

Gene IDs

7031

References

References

1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.

2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.

3. Marchbank T, Westley BR, May FE, et al. 1998. J Pathol, 185: 153-8.

4. Wang DG, Liu WH, Lundy FT, et al. 1998. J Pathol, 184: 408-13.