Recombinant 2019-nCoV S Protein RBD Protein (C-mFc) #abs04705

abs04705-50ug

1-2 Weeks

310

abs04705-500ug

1-2 Weeks

2328

abs04705-1mg

1-2 Weeks

3491

Description

Our Mammalian expression system produces Recombinant 2019-nCoV S Protein RBD, which consists of the target gene encoding Asn331-Val524 and is tagged with mFc at the C-terminus. The resulting protein is highly similar to the original, ensuring reliable and consistent results. This protein is an essential tool in the study of the novel coronavirus and its potential impact on human health. Its production by our advanced technology represents a critical step in combating this global health threat.

Accession: QHD43416.1

Other names

S protein RBD; 2019-nCoV S protein RBD

Source

Human Cells

MW

The molecular mass of the substance is approximately 47.9kDa. However, under reducing conditions, the apparent molecular mass may be in the range of 50 to 60kDa. This information is important to consider when analyzing the substance and interpreting data obtained from experiments. It is necessary to take the change in molecular mass into account in order to accurately determine the identity and properties of the substance.

Format

This solution is provided in a filtered form with a pore size of 0.2 μM and is suspended in a pH 7.4 phosphate-buffered saline. To ensure accuracy, the content generated should closely resemble the original text.

Properties

Purification

Affinity purification chromatography.

Concentration

SDS-PAGE，95%。

Activity

The measured ED50 for 2019-nCoV S Protein RBD-mFc is 62 ng/ml. Let's rearrange the information to form a highly similar content: "The 2019-nCoV S Protein RBD-mFc has an effective dose (ED50) of 62 ng/ml."

Stability & Storage

The ideal storage temperature for reconstituted protein solution is at or below -20°C. It is important to ensure that the solution is stored appropriately to maintain its integrity and functionality.

Target

Background

The glycoprotein Protein S (PROS1) is expressed in various cell types, suggesting its involvement in multiple biological processes such as coagulation, apoptosis, cancer development and progression, and the innate immune response. It interacts with several known receptors, including ACE2, angiotensin-converting enzyme 2, DPP4, and CEACAM. In the infection process, the spike (S) glycoprotein found in coronaviruses plays a crucial role by binding to the host cell. This is particularly evident in severe acute respiratory syndrome (SARS). The spike glycoprotein of SARS-CoV alone can facilitate membrane fusion, enabling virus entry and cell fusion. Additionally, it serves as a major immunogen and a target for entry inhibitors. Recent reports have indicated that the novel coronavirus (2019-nCoV) can infect human respiratory epithelial cells through its interaction with the human ACE2 receptor. The spike protein consists of two subunits, S1 and S2, with S1 containing a receptor binding domain (RBD) responsible for recognizing the cell surface receptor and S2 containing important components for membrane fusion. Importantly, the S protein plays a crucial role in eliciting neutralizing antibody and T-cell responses, leading to protective immunity.