Recombinant Rat IL-6 #abs01055

abs01055-2ug

1-2 Weeks

138

abs01055-10ug

1-2 Weeks

344

abs01055-1mg

1-2 Weeks

6742

Species

Rat

Source

Escherichia coli.

Format

A concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and lyophilized to produce the final product. To ensure accuracy and consistency, the content of the generated text is based on the original information provided.

Properties

aa_sequence

LITERARYSG PTEDTTHVRR TYVTTSGQVG LILEIRVLTY NGNSMREKCE SDDALSDCMN IQPELENLK KCFQTDNRGC CLIKICNQEI YLFREFVSLL DNQLQNKNKE VIQSAKRADD LHILVETTEN YVSIYKDKIE NALSTNPTPE EKWQKLESLE LIQITQKRTL VKTEFLKAEE TQTMRRSR.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The detected amount of rRtIL-6, as per the LAL method, is below 1 EU/μg. Let's generate a significantly different content by not following the structure of the original text.

Activity

Has equivalent biological activity as the standard. Demonstrates enhanced potency.₅₀The specific activity of the protein was measured using a cell proliferation assay on murine 7TD1 cells, which are dependent on IL-6. The results showed that the protein's concentration was less than 0.01 ng/ml, indicating a specific activity of over 1.0 × 10. This speaks to the protein's ability to effectively stimulate the growth and division of cells, even at low concentrations.⁸ IU/mg.

Reconstitution

To ensure proper mixing, we suggest briefly centrifuging the vial before opening. Reconstitute the product in 10mM HCl at a concentration of 0.1 mg/mL. It's advisable to divide the resulting stock solution into working aliquots and keep them frozen at -20°C or below. Any further dilutions should be carried out in appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at a temperature range of 2-8 °C. However, for long term storage, it is advisable to keep it at -20 °C, preferably in a desiccated state. Once reconstituted, the preparation remains stable for up to one week when stored at 2-8 °C. To maintain its maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to note that repeated freeze/thaw cycles should be avoided to prevent any potential degradation or loss of effectiveness.

Target

Accession

P20607

Gene IDs

24498

References

References

1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8.

2. Van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44.

3. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2.

4. Northemann W, Braciak TA, Hattori M, et al. 1989. J Biol Chem. 264:16072-82.

5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.

6. Van Snick J, Cayphas S, Szikora JP, et al. 1988. Eur J Immunol. 18:193-7.