Recombinant Human C-X-C Motif Chemokine 14/CXCL14 #abs04139

abs04139-10ug

1-2 Weeks

179

abs04139-50ug

1-2 Weeks

535

abs04139-500ug

1-2 Weeks

1879

abs04139-1mg

1-2 Weeks

2561

Description

Our E.coli expression system is used to produce Recombinant Human C-X-C Motif Chemokine 14 through the expression of the target gene that encodes Ser35-Glu111. We ensure that the final product is highly similar to the intended protein.

Other names

CXCL14, also known as C-X-C Motif Chemokine 14, Chemokine BRAKm, MIP-2G, Small-Inducible Cytokine B14, MIP2G, NJAC, or SCYB14, is a chemokine that plays a crucial role in various biological processes. This protein is known for its ability to regulate immune cell migration and activation, as well as its involvement in inflammatory responses.
CXCL14 is a member of the C-X-C chemokine family and is produced by various cell types, including macrophages, keratinocytes, and fibroblasts. It acts as a chemoattractant for immune cells, such as T cells and monocytes, and facilitates their recruitment to sites of inflammation or injury.
The expression of CXCL14 is regulated by various factors, including cytokines, growth factors, and microbial products. It has been implicated in several physiological and pathological conditions, such as cancer, wound healing, and autoimmune diseases. In cancer, CXCL14 has been found to have both tumor-suppressive and tumor-promoting effects, depending on the tumor type and microenvironment.
CXCL14 exerts its biological effects by binding to specific cell surface receptors, including CXCR4 and CXCR7. This interaction triggers downstream signaling pathways that regulate cell migration, proliferation, and survival.
Research on CXCL14 is ongoing, and its role in various diseases and biological processes is still being elucidated. However, its diverse functions and regulatory mechanisms make it an intriguing molecule for further investigation and potential therapeutic targeting.

Source

Escherichia coli.

Format

The solution containing 20mM TrisHCl and 1M NaCl with a pH of 8.5 was filtered through a 0.2 μm filter and then subjected to lyophilization. A highly similar content can be created by rearranging the information provided, but the language model used to generate the content would be significantly different from the original text.

Properties

aa_sequence

I apologize, but it seems that the provided content consists of random letters and does not convey any information. Can you please provide a meaningful input so that I can generate a similar response?

Concentration

The determination of greater than 95% was achieved through the use of SDS-PAGE reduction. It was found that the sample had a high level of similarity to the original content, indicating a strong consistency with the initial data. By rearranging the original information, we were able to generate this new content while preserving the key details.

Endotoxin_level

The LAL test results show that the level of endotoxins is less than 0.1 ng/μg (1 IEU/μg). This indicates that the product is free from harmful endotoxins that could cause adverse reactions.

Activity

The capacity to trigger calcium flux in THP1 human acute monocytic leukemia cells, when subjected to prostaglandin E2 treatment, serves as a measure of efficacy. Typically, the half-maximal effective dose (ED50) for this response ranges from 1.0 to 10.0 ng/ml. Let's produce a highly similar version of the content by reorganizing the information provided. However, please note that it is essential to generate content that differs significantly from the original text as per your request.

Reconstitution

To ensure proper handling of lyophilized protein, it is important to remember a few key steps. First and foremost, always centrifuge tubes prior to opening, and avoid mixing by vortex or pipetting. Secondly, it is not recommended to reconstitute the protein to a concentration less than 100 μg/ml. To dissolve the lyophilized protein, use ddH2O. Lastly, it is important to aliquot the reconstituted solution to minimize the number of freeze-thaw cycles. By following these guidelines, you can ensure maximum effectiveness of your lyophilized protein.

Stability & Storage

The stability guidelines for lyophilized protein storage indicate that temperatures below -20°C are suitable, but the protein can also be stored at room temperature for up to three weeks. On the other hand, reconstituted protein solutions should be stored between 4-7°C and remain viable for a period of 2-7 days. If aliquots of the reconstituted samples are prepared, they can be stored at temperatures below -20°C for a longer duration of three months. It is essential to adhere to these storage recommendations to ensure the preservation and integrity of the protein.

Target

Background

Human Chemokine (C-X-C Motif) Ligand 14 (CXCL14) is constitutively expressed in certain normal tissues but is reduced or absent from many established tumor cell lines and human cancers. CXCL14 is known to be a chemoattractant for monocyte and dendritic cells. CXCL14 inhibits angiogenesis and exhibits antimicrobial activities. Mature human and mouse CXCL14 differ by only 2 amino acid residues.

Accession

O95715