Western Primary&Secondary Antibody Removal Solution(Slightly Alkaline)

Western stripping fluids are also commonly referred to as membrane regeneration fluids or Western Stripping buffer. These fluids are essential for the reuse of membranes used during Western blotting experiments, especially in cases where the detection of reference or housekeeping proteins is also required.
After the primary and secondary antibodies have bound to the target protein on the membrane, chemiluminescence detection is carried out. However, in cases where reference proteins such as GAPDH or β-actin need to be detected as well, stripping buffer can elute the primary and secondary antibodies, allowing for easy reuse of the same membrane.
It should be noted that the repeated use of the same membrane can lead to reduced protein signaling, rendering Western stripping fluids unsuitable for repeated use after a certain number of cycles. Typically, a membrane can be reused for two to three times before stripping buffer ceases to be effective.
Primary and secondary antibodies can be completely removed using primary and secondary antibody removers, allowing the membrane to be easily reused for the detection of different proteins. This method not only saves time and effort compared to re-running an SDS-PAGE gel, but it also eliminates errors that may occur from re-loading, thereby improving the comparability of results.
The Western primary and secondary antibodies remover, which is weakly alkaline, is designed to remove the binding of primary and secondary antibodies in Western blotting without any protein loss from the membrane. This reagent relies heavily on its alkalinity to achieve this, making it suitable for protein membranes detected by Western chemiluminescence, particularly PVDF membrane, and nitrate cellulose membrane. However, this remover cannot be utilized for Western tests that are performed with non-chemiluminescent reagents like DAB, NBT/BCIP.

Notes:
If you are planning to use horseradish peroxidase labeled antibodies, then you must prepare the blocking solution with 5% skimmed milk powder. On the other hand, if you are working with alkaline phosphatase labeled antibodies, then the blocking solution must be made using casein. It is essential to select the correct blocking solution to ensure that the antibodies work efficiently and provide accurate results. Therefore, it is crucial to pay close attention to the specific requirements of the labeling method you are using and ensure that the blocking solution is prepared accordingly.
The Western primary and secondary antibody remover that uses a weak alkaline solution is particularly effective in regenerating PVDF membranes. However, when it comes to regenerating nitrate cellulose membranes, it is not as effective as it is with PVDF membranes. This finding highlights the importance of choosing the appropriate remover for specific types of membranes. It also emphasizes the need to consider the unique properties of each type of membrane to achieve optimal results when regenerating them.
The Western primary and secondary antibody remover, which has a weak alkaline formulation, is specifically designed to effectively remove primary and secondary antieluent following the detection of ECL and other chemiluminescent fluids. However, it should be noted that this particular reagent cannot be used on Western tests that utilize non-chemiluminescent agents like DAB or NBT/BCIP. So, it is imperative to bear that in mind while selecting the appropriate reagents for Western testing.
4, Western primary and secondary resistance remover (weak alkaline) is slightly corrosive, please pay attention to protection when using.
5. For your safety and health, please wear lab clothes and disposable gloves for operation.