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DAB Chromogenic Kit (Brown Yellow, WB)

DAB Chromogenic Kit (Brown Yellow, WB)

Please note that the price mentioned above is only for reference purposes. To obtain the actual price, kindly get in touch with our sales representative, Vecent. It is important to clarify the details with Vecent in order to avoid any confusion. Thank you for your understanding.

Description

SKU-Pack SizeAvailabilityPrice
abs9212-1kitIn stock$90.00

Please note that the price mentioned above is only for reference purposes. To obtain the actual price, kindly get in touch with our sales representative, Vecent. It is important to clarify the details with Vecent in order to avoid any confusion. Thank you for your understanding.


Description

Description

DAB (3,3'-diaminobenzidine) is a crucial substrate for peroxidase and plays a significant role in various analytical techniques such as spot detection, Western Blot, and immunohistochemistry. When catalyzed, it produces brown-yellow, water-insoluble products that can be easily observed visually. This unique property makes it suitable for staining procedures in these applications. Hence, DAB is widely utilized in research and diagnostics to enhance the detection and analysis of target molecules.

Composition:
Color solution A: DAB concentrate (40×) 3ml
Color solution B: H2O2 concentrate (40×) 3ml
Buffer solution C, with a concentration of TBS (40×), should be prepared by adding 3ml of the buffer solution.

 
Usage
To ensure thorough incubation in the peroxidase detection system, it is recommended to perform four washes with TBS or PBS for 5 minutes each. This will effectively remove any excess materials and optimize the detection process.
To prepare the film, start by adding 1 drop each of A, B, and C to 2ml of distilled water. Mix the solution thoroughly before adding it to the film. The color development process usually takes around 1-30 minutes at room temperature. If no background color appears, the color development can be extended. After the desired color is achieved, it is important to wash the film thoroughly with distilled water to stop the reaction.
4. Look and take pictures.
Storage Temp.
Stored at -20℃ for 1 year


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