Serum Free Cell Cryovials Plus

Product features:

1, natural ingredients, safe and non-toxic

2, ready-to-use, easy to operate, reduce pollution

Without using cooling procedures, the sample was placed directly in the -80℃ refrigerator. I would like to generate a highly similar content by rearranging the provided information. Please note that the context will remain the same but the wording might be different.

DMSO content: 10%

Endotoxin: 1 EU/ml

Prepare the cells :
Before proceeding, it is crucial to verify and validate that the cells have not been contaminated by fungi, bacteria, or mycoplasma. Please ensure that there is no presence of any of these contaminants within the cells.
The cells were cryopreserved when they were at their most optimal stage of growth - the logarithmic phase with a high rate of proliferation. This ensured that the cells would be stored in their most viable state for future use.
To determine the number of cells that need to be frozen, certain steps must be followed. Firstly, suspension cells should be centrifuged at a speed of 180g for a duration of 5 to 10 minutes. This step helps in removing the medium from the cells. Secondly, adherent cells need to be digested and counted separately. Once the counting process is complete, these cells should also be centrifuged at 180g to remove the medium, resulting in the formation of cell precipitation. By following these steps, a comprehensive count of the cells can be obtained for the freezing procedure.

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Note: The available cryopreservation density is 1~10× 10⁶ /ml, the recommended cryopreservation density is 2~5× 10 < sup > 6 < / sup > / ml. The freezing volume was 0.5~1ml/ tube.
2. Absorb appropriate amount of LC1601 resuspended cells, and the product should be pre-cooled at 2~8℃ before use. Cells should be resuspended immediately and repackaged into cryopreservation tubes. < BR />3. The cells after resuspension should not be allowed to stand at room temperature or 2~8℃ for more than 10 min. The cryopreservation tube should be moved into the -80℃ refrigerator as soon as possible, and no program cooling measures or program cooling devices should be used. If it cannot be immediately moved to the -80 ° C refrigerator, at least first should be moved to the environment below 0 ° C (such as -20 ° C refrigerator) for a short period of about 10 minutes, also do not take the program cooling.
4, -80℃ refrigerator overnight after the cryo-storage tube, if long-term storage, can be transferred to the liquid nitrogen tank for preservation. For short-term use, it can be stored at -80℃ for several months. The preservation time and effect vary with cell types. < span style = "box-sizing: border-box; color: RGB (74, 74, 74); line-height: 22px; font-size: 14px! Important; white-space: inherit! Important;"
2, reference C3A (human immortalized hepatocytes) to use this cryopreserve solution, 1× 10⁶/ml/ tube was stored at -80℃ for 12 months after recovery, the immediate survival rate > 90%.

1. Prepare the complete culture medium at room temperature or 37℃ with a volume 9 times the volume of the frozen liquid.
2. Take out the frozen tube in liquid nitrogen or -80℃, put it in a water bath at 37℃ and shake it gently. When the ice is about the size of a grain of rice, quickly remove it from the bath.
3. Immediately absorb the cryopreserved solution into the complete medium, gently blow for 3-5 times and mix well. Then, the cells were centrifuged at 180g for 5~10 minutes to prepare for cell precipitation.

1. After adding cells with this product, the storage time at room temperature should be reduced as far as possible, and the storage should be moved to -80℃ as soon as possible.

2. For the cryopreservation of stem cells (ES cells) and primary cells, it is recommended that the cells to be cryopreserved should be experimentally cryopreserved and cultured for at least one week before use, and the effect should be confirmed before mass cryopreservation.

3. This product contains 10%DMSO. For some cells sensitive to DMSO, it is suggested that preliminary experiments should be carried out first to confirm the effect before formal use in large quantities.

4. For new cell types that have not been preserved, it is recommended to freeze some cells in serum containing cryopreservation solution to avoid possible accidents when using this product.

5, the use of frozen liquid should be aseptic operation in the ultrapure workbench.

6. For your safety and health, please wear lab clothes and disposable gloves for operation.