Mycoplasma Clearance Reagents

An effective solution for combating mycoplasma contamination in cultured cells is the Mycoplasma Removal Agent. This reagent serves a dual purpose - to eliminate existing mycoplasma contamination and to prevent future contamination. Derived from a natural antibiotic obtained through rigorous screening, this agent is highly potent against mycoplasma while maintaining a low concentration that is non-toxic to cells. It functions similarly to widely used antibiotics like penicillin and streptomycin, without interfering with various cell detection methods. Its efficacy extends to various species of mycoplasma, including Mycoplasma orale, M. arginini, M. hyorhinis, and Acholeplasma laidlawii. Rest assured, with the Mycoplasma Removal Agent, your cell cultures can be safeguarded from this pervasive contamination.

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1. Preparation of storage solution:
To prepare a 10 mg/ml stock solution of mycoplasma removal reagent, measure out an appropriate amount of the reagent and dissolve it in water. Once the solution is prepared, it should be aliquoted and stored at a temperature of -20°C to maintain its stability. If the solution is not going to be used for an extended period of time, it can be transferred to a freezer set to -80°C for long-term storage. It is important to ensure that the reagent is stored correctly to maintain its efficacy for future use.
To avoid mycoplasma contamination or hinder the growth of mycoplasma, there are a few measures that can be taken. These include ensuring good laboratory practices, using proper disinfectants, filtering cell culture media and regularly monitoring cell cultures for mycoplasma. By taking these precautions, we can prevent the spread of mycoplasma infections and maintain the integrity of our research.
To ensure prevention of mycoplasma contamination or inhibition of mycoplasma proliferation, it is recommended to add an appropriate amount of mycoplasma removal reagent stock to the cell culture medium. The final concentration should be within the range of 0.01-0.5μg/ml. Typically, the recommended concentration is either 0.2μg/ml or 0.5μg/ml. By adding the aforementioned dosage of mycoplasma scavenging reagent, the growth and spread of mycoplasma can be effectively controlled.
3. Remove contaminated mycoplasma:
(1) For cells found or suspected of mycoplasma contamination, add an appropriate amount of mycoplasma removal reagent stock to the cell culture medium to make the final concentration 0.5-250μg/ml. It is recommended to try the 4 concentrations of 25, 50, 100 and 250μg/ml in sequence. After culturing in the cell culture medium supplemented with the aforementioned dose of mycoplasma removal reagent for 1 week, it was detected whether there was still mycoplasma contamination. If there is still mycoplasma contamination, the working concentration of mycoplasma removal reagent can be increased as appropriate.
(2) If there is mycoplasma contamination in the cell culture medium or pancreatin, the above step 2 can be used to prevent or inhibit mycoplasma contamination.

1. Mycoplasma removal reagent is irritant to human body, please be careful when operating and pay attention to proper protection to avoid direct contact with human body or inhalation.
2. The reagent can effectively inhibit or remove a variety of mycoplasma, but there is no guarantee that it can inhibit or remove any type of mycoplasma encountered in the actual experimental operation.And in the case of long-term use of this reagent, it is not ruled out that there will be mycoplasma resistance.In cases where this reagent does not work well, the mycoplasma removal reagent (ABS9254) from Epsilon may be considered.
3. It is only used for scientific research by professionals, and shall not be used for clinical diagnosis or treatment, or for food or medicine.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

Store at 4°C, valid for one year. After being prepared as a solution, store at -20°C.

Mycoplasma Removal Agent

It is soluble in water, and the solubility in water is about 15mg/ml.