Mayer's Hematoxylin Stain

In the world of nuclear staining, Haematoxylin reigns supreme as the go-to dye. Derived from the Haematoxylin campechianun plant, this precious substance found its spotlight in the year 1865 when Bohmer first utilized it to stain biological samples. Over time, various minds tinkered with the formula, bringing forth modifications that enhanced its efficacy. One such innovator was Mayer, who in 1903, made notable changes to the Haematoxylin recipe, paving the way for others to follow suit. Even to this day, staining techniques bearing the names of Harris, Gill, Mayer's, and Weigert's continue to hold their ground in laboratories across the globe.
Before this magnificent dye is applied, a crucial step comes into play: oxidation. Metal ions, in the form of mordants, undertake this task. Aluminum and iron salts are among the commonly employed mordants, aiding in the transformation of hematoxylin into its vibrant, color-inducing state. This strategic process ensures that the dye adheres to the desired target, enabling scientists to unlock valuable insights within the realm of biology and medicine. The enduring presence and utilization of this staining technique testify to its enduring importance in the scientific community.

Hematoxylin dyes are divided into two main categories, progressive and regression, based on their concentration. Progressive staining methods, like Mayer's hematoxylin, are done with lower dye concentrations and target the nucleus for staining as opposed to the chromatin. However, if the staining is prolonged, progressive staining can appear similar to regression staining. On the other hand, regression staining methods, such as Harris hematoxylin, produce intense staining of the tissue components, including the nucleus and cytoplasm. In order to achieve the correct coloration, excessive dye must be removed, and appropriate differentiation and decolorization will reveal the nuclear staining without staining the chromatin structure.

Mayer's hematoxylin solution is a widely used method for restaining cell nuclei after undergoing immunohistochemical and cytochemical staining. It is particularly useful for hydrochloric acid ethanol differentiation or ethanol differentiation, which can damage the cytoplasm. Additionally, it can also be incorporated into standard hematoxylin-eosin (HE) staining. Overall, this solution plays a vital role in accurately and effectively staining cells for a variety of research purposes.

The tissue sections undergo a process called hematoxylin staining, which results in a distinct blue coloration. After staining, the tissue sections may appear in shades of purple or reddish-purple. To achieve the blue color, the stained sections are treated with various alkaline solutions like warm tap water (if slightly alkaline), dilute ammonia, Scott's tap water substitute, or lithium carbonate. This alkaline treatment transforms the purple or reddish-purple stained sections into a characteristic blue hue.

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1. 95%：1.1 95mL5ml；1.2 、；1.3 Mayer15；1.4 15；1.5 30；1.6 ，95%95%（BF057）30；1.7 Eosin Y 30~60；1.8 2，2；1.9 。
2. ：2.2 ；2.3 Mayer1~5；2.4 ，；2.5 ；2.6 ，。，、、。
：，。（），。

Room temperature, sealed away from light, valid for 12 months.

1. Mayer's hematoxylin dye solution should be filtered before each use.
2. The dipping time is the approximate estimated time. The dyeing time can be adjusted according to personal preference. Repeated use of the dye solution will make it lose its dyeing ability, which will lead to the extension of the dyeing time. At this time, a new dye solution should be used.
3, can use warm tap water instead of dilute alkaline solution. This will shorten the time required for the dyeing process. Before dyeing eosin, if using dilute alkali solution, it must be rinsed with tap water for 2 to 3 minutes.
4, some tap water is acidic, not suitable for the process of "blue" experiment. If tap water is acidic, dilute it with an alkali solution.
5, the core is purple or brownish red, indicating that the "blue" is not sufficient.
6. Nuclear staining can be masked if eosin is overstained. To increase eosin differentiation, prolong the time of alcohol or use alcohol with higher water content. The correct degree of eosin staining can be obtained by adjusting the time of alcohol.
7. Filter and stain before use. Ethanol, xylene/xylene substitute should be replaced daily.
8. It is not recommended to mix different batches of hematoxylin or eosin dyes.
9. Avoid carrying excess water into Mayer's hematoxylin dye.
10. Positive controls should be used for each run.
11. When using this kit for the first time, it is recommended to take 1 or 2 samples for pre-experiment.
12, for your own safety, before using the reagent, please do a good job of protection, such as wearing lab clothes, gloves, etc.

Mayer's hematoxylin dye