EDTA Antigen Retrieval Solution(50×)

Paraformaldehyde, formaldehyde, and other aldehyde fixatives can cause cross-links between proteins, which can mask the antigenic sites and weaken the staining signals during immunostaining. This can even lead to false positive staining results. To overcome this problem, EDTA Antigen Retrieval Solution (50×) is widely used. It contains EDTA, which effectively removes the cross-links between proteins caused by aldehyde fixatives, and exposes the antigen epitopes in paraffin samples, improving the immunostaining effect significantly.
Typically, paraffin sections require antigen retrieval while frozen sections do not. However, studies have shown that even the immunostaining effect for frozen sections can be significantly improved through antigen retrieval. It is important to note that whether it's frozen sections or cell slides, as long as the samples are fixed with paraformaldehyde or other aldehyde fixatives, antigen retrieval will remove the cross-links between proteins and expose the epitopes, leading to a better immunostaining effect.

This particular method is ideal for usage with paraffin sections, as well as other types of samples like frozen sections. By rearranging the original content, a highly similar message can be conveyed. However, it's important to note that the text should be generated based on the given information rather than using ChapGPT to produce similar content.

1. Paraffin section:
In the process of deparaffinization, the sections undergo a series of steps to remove the paraffin. Initially, they are immersed in xylene for two cycles of five minutes each. Subsequently, the sections are placed in anhydrous ethanol for two cycles of five minutes each. To further eliminate any remaining paraffin, the sections are then transferred to 90% ethanol for two cycles of five minutes each. This is followed by a five-minute immersion in 70% ethanol. Finally, the sections are washed in distilled water, undergoing two cycles of five minutes each. These sequential steps ensure the effective deparaffinization of the sections, preparing them for further analysis.
To retrieve antigens, immerse the tissue slices in a preheated antigen retrieval solution (1×) and heat them at a temperature of 95-100°C for approximately 10-20 minutes. It's important to find the optimal heating time based on the specific samples and target proteins being studied. Take caution when using a microwave for heating to prevent any solution spillover. After heating, let the slices cool down to room temperature within about 20-30 minutes. Then, wash the slices with immunostaining washing solution 1-2 times for 3-5 minutes each. Following these steps, you can continue with subsequent immunostaining procedures like blocking.
2. Frozen sections: Wash the sections with immunostaining washing solution for 5 minutes. The process of antigen retrieval is like that of paraffin section.
3. Antigen retrieval of other samples can refer to the steps of paraffin section.

1. For your own safety, please protect yourself before using reagents, such as wearing lab coats and gloves.

2. The EDTA antigen retrieval solution (50×) must be diluted 50 times with redistilled water or MilliQ water before use to prepare the antigen retrieval solution (1×).

Store at 2~8° C. If you do not use it for a long time, please store it at -20°C. The validity period is 12 months.

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