Coomassie Brilliant Blue Staining Kit(conventional Type) R250

The Commassie Blue Staining Kit is a time-tested, classic method for staining and decolorizing protein electrophoresis gels such as SDS-PAGE or non-denaturant-resistant PAGE. Coomassie Blue R250 is used as the dye in this kit, and it is perfect for routine use. Additionally, it can be used to detect residual protein on the PAGE glue after Western transfer. With its ability to produce reliable and reproducible results, the Commassie Blue Staining Kit is an essential tool for any proteomics research lab.

Composition:

The Coomath bright blue stain comes in two different packaging options. One is a 100mL bottle, and the other is a larger 500mL bottle of Coomath bright blue stain decoloring solution. These products are a great addition to any laboratory or scientific research setting. They are designed to be easy to use and highly effective at achieving accurate results. So, whether you are a professional researcher or just someone interested in science, these products are sure to meet your needs and exceed your expectations!

liquid

Here is a rearranged version of the content:
For conventional staining and decolorization method:
1. After electrophoresis, fully cover the gel with an appropriate amount of Coomassie bright blue staining solution.
2. Place the gel on a horizontal or side shaking table and shake it slowly at room temperature for at least 1 hour. Adjust the dyeing time based on the gel thickness and temperature. When the gel color matches the staining solution and becomes almost invisible, it indicates complete staining.
3. Discard the staining solution, which can be recycled and reused 2-3 times.
4. Ensure the gel is fully covered with Coomassie bright blue decolorization solution.
5. Shake the gel on a shaking table at room temperature for 4-24 hours. Replace the decolorization solution 2-4 times until the blue background is removed and the desired protein bands are clearly visible. Protein bands usually appear after 1-2 hours of decolorization. Be cautious not to bleach the protein strips too much, as it may lighten their color.
6. After decolorization, store the gel in water for subsequent procedures like photography. However, be aware that water storage may cause gel dissolution. To prevent dissolution, store the gel in water containing 20% glycerin or prepare a dry adhesive for long-term storage.
For rapid dyeing and decolorization method:
1. After electrophoresis, place the gel in an appropriate amount of Coomassie bright blue staining solution and heat it in a microwave oven until close to boiling or just boiling. Avoid boiling if the gel concentration is less than 10% to prevent fragmentation.
2. Shake the staining solution at room temperature for 5-10 minutes.
3. Discard the staining solution, which can be recycled and reused multiple times.
4. Ensure full coverage of the gel with Coomassie bright blue staining and decolorization solution.
5. Heat the solution to near boiling or just boiling in the microwave and immediately stop heating.
6. Shake the gel on a shaking table at a high temperature decolorization solution for 5-10 minutes. Typically, clear protein bands are observed at this stage.
7. Replace the decolorization solution with fresh solution and repeat steps 5 and 6 until the blue background is removed completely and the desired protein bands are achieved.
8. After decolorization, the gel can be stored in water for subsequent photography. However, water storage may cause gel dissolution. To prevent dissolution, store the gel in water containing 20% glycerin or prepare a dry adhesive for long-term storage.

For your own safety, please do a good job of protection before using the reagent, such as wearing lab clothes, gloves, etc.

Store at 2-8℃ for 1 year.