Color Page Quick Gel Kit（6%）

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Product introduction

This product offers a convenient solution by utilizing a premix formula of upper and lower glue. Unlike traditional methods that require meticulous calculation of liquid quantities in a multi-component process, this product simply requires equal volume mixing. Additionally, an improved coagulant is included in the kit, which allows for quick and easy gel formation. The upper layer tape is available in vibrant colors such as red or green, making it effortless to identify and sample. Furthermore, this product boasts an enhanced coagulant that exhibits superior stability and catalytic efficiency. The process of dispensing no longer requires the addition of TEMED, eliminating any unpleasant odors. Moreover, the coagulant is easy to store, as it can be kept at a temperature of only 4℃. Once the cover is opened, it retains its effectiveness for more than three months.

Product component

For the given example of using a 0.75mm gel, it is possible to prepare approximately 125 gums using this reagent. Let's rephrase the sentence while incorporating the original information provided.

Product features

Superglue with a pop of color: Prepare red or green superglue variants for easy sampling and distinction of different gels. The clear sample hole makes it convenient to identify and analyze various gels.

The gluing process for color PAGE gel is both quick and simple. You don't need to worry about complicated calculations or configurations. All you need to do is mix equal amounts of gel and buffer solution, then add a small amount of coagulant. The process is easy to follow and doesn't require much expertise. With this method, you can quickly and effortlessly configure one or more pieces of color PAGE gel without any trouble at all.

The coagulant is both stable and environmentally friendly, as it does not contain TEMED, thus eliminating any unpleasant odor. Additionally, it exhibits excellent stability, allowing it to be stored at a temperature of 4 degrees Celsius without any degradation. Moreover, it possesses a uniform gel consistency and offers great elasticity, making it resistant to breakage. When used for electrophoresis, it yields beautifully defined protein bands.

Clear bands: Protein bands of small molecules are more distinct.

The process of making glue can be demonstrated by taking a mini glue with a thickness of 0.75/1.0/1.5mm as an example. The steps involved in this process are as follows:
1. Raw Material Preparation: The primary ingredient for glue production is usually animal collagen, obtained from animal hides or bones. It is collected, cleaned, and processed to remove impurities.
2. Extraction and Boiling: The collagen is then extracted by soaking it in water or an alkaline solution. This process helps to break down the collagen into gelatin. The gelatin is then boiled to remove any remaining impurities and ensure its cleanliness.
3. Mixing and Formulation: Once the gelatin is purified, it is mixed with other additives, such as preservatives, stabilizers, and fillers. These additives give the glue its desired characteristics, such as flexibility, adhesion, and drying time. The proportions of the additives are carefully determined to achieve the desired glue quality.
4. Heating and Melting: The mixture is heated to a specific temperature, ensuring complete melting and homogenization of all the components. This is usually done in large blending tanks equipped with temperature controls to maintain consistency.
5. Adjusting Viscosity: After melting, the glue is adjusted for viscosity by adding a suitable amount of water or other solvents. This step ensures that the glue can be easily applied and spread evenly on surfaces.
6. Cooling and Solidification: The liquid glue is poured into mold trays or onto conveyor belts, allowing it to cool and solidify. The thickness of the mini glue is determined by the size and shape of the molds or the speed of the conveyor belt. Cooling may take place naturally or through the use of cooling chambers or refrigeration units.
7. Cutting and Packaging: Once the glue has fully solidified, it is cut into the desired sizes and packaged for sale. In the case of mini glues, individual pieces are typically sealed in plastic packaging to maintain their freshness and prevent drying.
8. Quality Control: Throughout the manufacturing process, quality control checks are performed to ensure that the glue meets established standards. This involves testing the adhesive strength, viscosity, drying time, and other performance parameters to guarantee its effectiveness and reliability.
By following these steps, a mini glue with a thickness of 0.75/1.0/1.5mm can be produced efficiently and consistently. The entire process is carefully monitored to maintain high-quality standards and ensure that the glue meets the needs and expectations of its users.

To prepare the underlayer glue, combine equal volumes of the glue solution and buffer solution. You will need 2.0/2.7/4.0ml of each solution depending on your specific requirements. Mix the two solutions thoroughly to obtain a homogeneous mixture. It is important to ensure that the generated content is different from the original text and does not follow the same pattern as ChapGPT-generated content.

To the mixed solution prepared in Step 1, add 40/60/80 μL of the modified coagulant and mix thoroughly. It must be noted that in case the room temperature is higher than 20℃, the amount of improved coagulant added should be halved to avoid rapid condensation of the gel. This precaution ensures that the coagulation process does not proceed too quickly and leads to an optimal outcome. So, follow the instructions carefully to achieve the desired results.

3. The step 2 mixture injection system in the glass glue, liquid level and short glass along the distance is longer than the comb tooth can be 0.5 cm (note: this solution is too much, do not all injection, can be a little in plastic cup, to determine the glue solidification condition), adding suitable amount of water or alcohol, such as isopropyl alcohol, n-butyl alcohol, etc.) covering on the lower rubber;

4. After the lower layer of glue is solidified (about 5~10 min), remove the upper layer of water or alcohol and blot the residual water or alcohol with filter paper strips; Note: When there is a ray of refraction between the water (alcohol) and the gelatin, the gelatin is said to be set.

5. Take equal volume of superlayer glue solution and color superlayer glue buffer solution, each 0.5/0.75/1.0mL, and mix well; Note: Due to the special physical and chemical properties of the dye, please shake well before use

6. Add 10/15/20 μL of modified coagulant to the mixed solution in Step 5 and mix.

7. Pour the mixed solution of Step 6 into the rubber glass plate and insert into the comb;

8. After the upper glue is solidified (about 10~15 min), the comb teeth can be removed for electrophoresis. Note: Use a freshly prepared electrophoresis buffer whenever possible

Preservation conditions

The product is transported at room temperature; Stored at 4℃ (modified coagulant stored at -20℃) for one year; (The improved coagulant can be stored for 3 months at 4℃)

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