Cell Cryopreservation Solution(Containing Serum)

solution

Procedure of cell cryopreservation:
The conventional methods were used to collect the adherent cells or suspension cells of logarithmic stage in test tubes.
To determine the amount of cells to be frozen, we took into consideration the density of the cultured cells as well as the size of the cryopreservation tube. By considering these factors, we were able to determine the appropriate number of cells to be frozen in each tube for optimal preservation.
To collect the cell precipitates, the recommended amount of cell suspension was loaded into a centrifugal tube and centrifuged at a speed of 1000 revolutions per minute for a duration of 5 minutes. Once the centrifugation process was complete, the supernatant in the tube was emptied completely, leaving behind the cell precipitates collected at the bottom.
To prepare cells for cryopreservation, a suitable amount of cryopreserve solution should be added into the centrifuge tube. This will ensure that the cell concentration is approximately 1× 10. It is important to carefully measure the solution and adjust the concentration accordingly, as this will impact the success of the cryopreservation process. By following these steps, you can successfully preserve your cells for future use.⁶，。-1×10^7/ml。，，。ChapGPT。
To properly cryopreserve cells, it's essential to follow a strict protocol. First, you need to prepare labeled cryopreserved tubes. Then, take the cell mixture in the centrifuge tube and divide it into each labeled cryopreserved tube. Once you finish dividing, seal and mark each tube, following the conventional operation process.
Next, it's time to freeze the tubes properly. It's best to cool the tubes gradually, step by step. Start by cooling them to 4℃, followed by -20℃, and then finally to -80℃. Once your tubes have reached -80℃, you can store them in a liquid nitrogen tank or program temperature reducer for maximum freezing efficiency. Follow these steps precisely to cryopreserve your cells successfully.
To quickly thaw frozen cells, take them out of the fridge and put them in a water bath set at 37℃. It's important to do this right away to avoid any delay in the thawing process.
Once the cell mixture in the cryobor had fully melted, a volume of 1mL from the cell culture was promptly introduced to the cryobor and thoroughly mixed with the cell components. The resultant mixture was then transferred to a centrifuge tube already containing approximately 5mL of the cell culture medium, followed by centrifugation for 5 minutes at 1000rpm.
3. Add appropriate amount of fresh culture medium, use pipette to slowly add to the cell precipitation, gently mix, and move the mixed cells to the prepared culture container.
4. Microscopic observation. After the cell state recovers, other studies or culture treatment can be carried out.

Stored at -20℃, valid for 2 years.

Cell Freezing Medium