Creatine Kinase Microplate Assay Kit

EC 2.7.3.2; Creatine kinase; CK

Quantification and Detection of Creatine Kinase Activity is an essential procedure in various clinical and research settings. This process involves measuring the levels of creatine kinase, an enzyme found in muscles and the brain, to assess the integrity and function of these tissues.
With the aim of quantifying creatine kinase activity, various tests and techniques have been developed. One commonly used method is the spectrophotometric assay, which relies on the reaction between creatine kinase and adenosine triphosphate (ATP). This reaction produces adenosine diphosphate (ADP) and phosphocreatine.
The spectrophotometric assay involves measuring the change in absorbance at a specific wavelength as the reaction progresses. By correlating this change in absorbance with known standards or controls, the activity of creatine kinase can be determined accurately.
Another method for quantifying creatine kinase activity is through the use of specific substrates that react with the enzyme. For example, the N-acetyl-L-cysteine creatine kinase (NAC-CK) method utilizes N-acetyl-L-cysteine as a substrate. This reaction leads to the production of a chromophore, which can be measured spectrophotometrically.
In addition to quantification, the detection of creatine kinase activity is also important. This is commonly achieved by using immunological techniques such as enzyme-linked immunosorbent assays (ELISA) or immunohistochemistry. These methods involve the use of antibodies that specifically bind to creatine kinase, allowing for its visualization and localization within tissues or cells.
Overall, the detection and quantification of creatine kinase activity are crucial for evaluating various medical conditions, including muscle and brain disorders. These methods provide valuable insights into the functioning and health of these tissues, aiding in the diagnosis and monitoring of diseases.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.