Aconitase Microplate Assay Kit

AcnB, also known as cis-aconitase or aconitase, is an enzyme that belongs to the EC 4.2.1.3 group of hydratases. It is involved in the hydrolysis of citrate(isocitrate) and the conversion of 2-methylaconitate. This process is an important step in the citric acid cycle, which is vital for cellular respiration. As a highly specialized enzyme, AcnB plays a crucial role in metabolic reactions and is essential for maintaining proper cellular function. Its ability to catalyze the conversion of cis-aconitate to isocitrate is critical for the production of ATP, and any disruption in its activity can lead to a range of metabolic disorders. Overall, AcnB is a critical enzyme in biological processes and has broad implications for human health and disease.

A The detection and quantification of aconitase activity is an important process for measuring the activity levels of this enzyme. Aconitase is an enzyme that is involved in the citric acid cycle, and its activity is often used as a marker for mitochondrial function.
To detect and quantify aconitase activity, researchers typically use spectrophotometric methods or enzyme assays. Spectrophotometric methods involve measuring changes in the absorbance of light at a specific wavelength, while enzyme assays involve measuring the conversion of a substrate to a product over time.
Both methods require careful preparation of samples and precise measurements to ensure accurate results. Researchers must also consider the sensitivity and specificity of the assay, as well as any potential interference from other enzymes or compounds in the sample.
Overall, the detection and quantification of aconitase activity is a crucial step in understanding mitochondrial function and metabolism. By accurately measuring aconitase activity, researchers can gain insights into the underlying mechanisms of diseases such as cancer, diabetes, and neurodegenerative disorders, and develop more effective treatments.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.