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Rabbit Anti-SMO Polyclonal Antibody#abs135454

Please note that the mentioned price is only for your reference. For detailed pricing information, we recommend reaching out to our seller, Vecent. Kindly contact Vecent for further assistance. Western blot analysis of extracts from HuvEc cells, using SMO antibody. This product is for research...

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Description

Catalog-specification	Delivery time	USD price
abs135454-100ug	1-2 Weeks	301.0
abs135454-50ug	1-2 Weeks	201.0

Please note that the mentioned price is only for your reference. For detailed pricing information, we recommend reaching out to our seller, Vecent. Kindly contact Vecent for further assistance.

Overview
catalog	abs135454
Other names	Protein Gx, also known as Frizzled family member 11 (FZD11), is a crucial receptor in the Smoothened frizzled family. It plays a significant role in G-protein signaling pathways. The human counterpart, SMO_HUMAN, is a precursor of the Smoothened homolog protein, responsible for seven transmembrane spanning receptor activity. The function of SMOH is highly conserved in various species, including the well-studied Drosophila fruit fly. Understanding the intricate mechanisms and interactions involving SMO is vital for unraveling its impact on cellular processes. By rearranging the provided content, we can gain insights into the fascinating world of SMO and its importance in the field of molecular biology.
Source	Rabbit
Specificity	The total SMO protein can be detected using the SMO Antibody, which specifically targets the endogenous levels of SMO in the sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Bovine;Chicken;Horse
Antigen	SMO
Application	For Western blotting, a dilution range of 1:500 to 1:1000 is recommended. Immunofluorescence and immunocytochemistry assays typically require a dilution of 1:100 to 1:500. For peptide ELISA, a dilution range of 1:20000 to 1:40000 is suitable. It is important to carefully optimize the dilution factors for each assay to achieve optimal sensitivity and specificity.
Immunogen	A synthesized peptide.
MW	86 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. It was purified to remove any impurities, providing a pure form of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, the product should be stored at a temperature of -20 °C for a duration of one year. It is crucial to prevent the product from undergoing multiple freeze and thaw cycles.
Storage buffer	The rabbit IgG solution is formulated in a pH 7.4 phosphate buffered saline with 150mM NaCl, 0.02% sodium azide, and 50% glycerol for optimal stability and longevity. When stored at -20°C, this solution remains stable for up to 12 months from the date of receipt. It's important to follow storage requirements to ensure optimal performance and efficacy.
Target
Background	G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed:19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	Q99835