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Rabbit Anti-POU3F3 Polyclonal Antibody#abs138608

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent. Western blot analysis POU3F3 using K562 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs138608-100ug	1-2 Weeks	301.0
abs138608-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent.

Overview
catalog	abs138608
Other names	Brain 1, also known as Brain-specific homeobox/POU domain protein 1 or Brn-1, is a transcription factor that plays a crucial role in brain development and function. It is classified as a class 3 POU domain protein and is closely related to Octamer-binding protein 8 (Oct-8) and POU domain protein 12 (POU3F3). The expression of Brain 1 is highly specific to the brain, hence its name. It is involved in regulating the expression of genes that are key for the development and maintenance of neural cells. In addition, Brain 1 is essential for the formation and maturation of neuronal circuits. It works by binding to specific DNA sequences and controlling the transcription of target genes. Studies in mice have shown that the absence of Brn-1 leads to severe brain abnormalities and impaired cognitive function. It is also believed to be involved in the pathogenesis of certain neurological disorders, such as epilepsy and schizophrenia. Brain 1 is part of a larger family of POU domain proteins, which are important regulators of gene expression in various tissues and organs. The POU domain is a conserved DNA-binding domain that allows these proteins to recognize and bind to specific DNA sequences. In summary, Brain 1 is a critical transcription factor for brain development and function. Its intricate regulation of gene expression is necessary for the proper formation and functioning of the brain. Further research on Brain 1 and its related proteins may provide valuable insights into neurodevelopmental disorders and potential therapeutic targets.
Source	Rabbit
Specificity	The total POU3F3 levels are detected by the POU3F3 Antibody, which specifically targets the endogenous expression of POU3F3. Let me rephrase this in a highly similar manner: The endogenous levels of total POU3F3 can be detected using the POU3F3 Antibody, which is designed to specifically recognize and measure the expression of POU3F3 protein in biological samples.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken
Antigen	POU3F3
Application	The recommended dilution for Western Blot (WB) ranges from 1:1000 to 1:3000, while for Immunohistochemistry (IHC) it is 1:200. For ELISA (peptide), the suggested dilution is between 1:20000 and 1:40000. Make sure to follow these guidelines to achieve optimal results.
Immunogen	A synthesized peptide derived from human POU3F3.
MW	50 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was carried out through peptide affinity chromatography. To obtain high purity levels, this method was chosen as it is highly effective in separating peptides from complex biological matrices.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).
Tissue specificity	Brain.
Celluar localization	Nucleus;
UniPort	P20264