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Rabbit Anti-PHKB Polyclonal Antibody#abs140510

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Description

Catalog-specification	Delivery time	USD price
abs140510-100ug	1-2 Weeks	301.0
abs140510-50ug	1-2 Weeks	201.0

Overview
catalog	abs140510
Other names	The human gene DKFZp781E15103, also known as FLJ41698, codes for a protein called Phosphorylase b kinase regulatory subunit beta, or KPBB_HUMAN. This protein plays a crucial role in cellular metabolism, specifically in the regulation of glycogen metabolism. It is a subunit of the phosphorylase kinase B enzyme, which helps break down glycogen into glucose for energy. Phosphorylase kinase B is composed of four subunits, and the beta subunit is one of them. Without this subunit, the enzyme cannot function properly and the body cannot effectively regulate glycogen metabolism. Mutations in the gene that codes for the beta subunit can lead to glycogen storage diseases, which are rare genetic disorders that affect the body's ability to regulate its glycogen stores and can cause a variety of symptoms. Overall, the role of the Phosphorylase kinase beta subunit in regulating glycogen metabolism is crucial for maintaining energy balance in the body and preventing metabolic disorders.
Source	Rabbit
Specificity	The total PHKB within the organism can be detected using the PHKB Antibody. This antibody can identify the native levels of PHKB present in the body accurately.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Zebrafish;Rabbit;Pig;Dog;Horse;Bovine;Sheep;
Antigen	PHKB
Application	We have different dilution ratios for various assays. For Western blotting, we recommend a dilution of 1:1000. For immunofluorescence and immunocytochemistry, the recommended dilution ranges from 1:100 to 1:500. In case of ELISA using a peptide, the optimal dilution range is between 1:20000 and 1:40000. These dilution ratios ensure accurate detection and measurement of the target protein in each assay.
Immunogen	A synthesized peptide derived from human PHKB.
MW	124kDa
Properties
Concentration	1mg/ml
purification	Utilizing the SulfoLink™ Coupling Resin, peptide affinity chromatography was employed in the purification of the antiserum. A highly similar content can be generated by rearranging this information, while ensuring that the original message remains intact.
Clonality	Polyclonal Antibody
Stability & Storage	Please ensure that the store is maintained at -20 °C for a period of one year without subjecting it to repeated freeze/thaw cycles.
Storage buffer	The Rabbit IgG is stored in a solution of phosphate buffered saline, with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to be stored at a temperature of -20°C. The solution is stable for up to 12 months from the date of receipt.
Target
Background	Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.
Posttranslational modification	Ser-701 is probably phosphorylated by PKA.Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
Celluar localization	Cytosol;Plasma Membrane;
UniPort	Q93100