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Rabbit Anti-PDPK1 Polyclonal Antibody#abs136060

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Description

Catalog-specification	Delivery time	USD price
abs136060-100ug	1-2 Weeks	301.0
abs136060-50ug	1-2 Weeks	201.0

Overview
catalog	abs136060
Other names	Phosphoinositide-dependent protein kinase 1 (PDK1), also known as hPDK1 or PDPK1_HUMAN, is a crucial protein kinase involved in various cellular processes. It is encoded by several genes, including MGC20087, MGC35290, OTTHUMP00000159109, OTTHUMP00000159110, OTTHUMP00000174525, and PDPK2P. PDK1 plays a vital role in mediating cell signaling pathways and regulating key biological functions. PDK1, also referred to as PkB kinase or PkB-like kinase 1, exhibits high similarity to the protein kinase B (PKB) family. Its importance lies in its ability to respond to phosphoinositide signaling and regulate downstream effectors. PDK1 is involved in the phosphorylation and activation of several protein kinases, enabling the transmission of intracellular signals. The intricate network of PDK1-mediated pathways contributes to various physiological processes, including cell growth, survival, metabolism, and differentiation. Dysregulation of PDK1 has been linked to numerous diseases, such as cancer, diabetes, and cardiovascular disorders. Therefore, understanding the functions and regulation of PDK1 has significant implications for therapeutic interventions. In summary, PDK1, or 3-phosphoinositide-dependent protein kinase 1, is a critical player in cellular signaling pathways. Its multiple gene variants, including hPDK1, MGC20087, MGC35290, OTTHUMP00000159109, OTTHUMP00000159110, and OTTHUMP00000174525, contribute to its diverse functions. PDK1's role as a regulator of protein kinases and its involvement in various biological processes underscores its importance in maintaining cellular homeostasis.
Source	Rabbit
Specificity	The antibody, PDPK1 Antibody, has the ability to identify the total PDPK1 protein present in the body at its natural levels. Utilizing this antibody allows for the detection and analysis of endogenous levels of PDPK1 protein.
Species Reactivity	Human;Mouse;Rat
Antigen	PDPK1
Application	For western blot analysis, a dilution range of 1:500 to 1:2000 is recommended. In terms of immunohistochemistry, a dilution range of 1:50 to 1:200 is appropriate. ELISA analysis of peptide samples can be performed with a dilution range of 1:20000 to 1:40000.
Immunogen	A synthesized peptide derived from human PDPK1.
MW	63kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for peptide affinity chromatography purification of the antiserum. The resulting content is highly similar to the original text but rephrased to avoid verbatim replication.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing. This will help maintain the integrity and quality of the product throughout its storage duration.
Storage buffer	The Rabbit IgG is suspended in phosphate buffered saline at a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The product has a stable shelf life of 12 months from the date of receipt.
Target
Background	Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.
Tissue specificity	Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.
Posttranslational modification	Phosphorylation on Ser-241 in the activation loop is required for full activity. PDPK1 itself can autophosphorylate Ser-241, leading to its own activation. Autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2 (By similarity). Tyr-9 phosphorylation is critical for stabilization of both PDPK1 and the PDPK1/SRC complex via HSP90-mediated protection of PDPK1 degradation. Angiotensin II stimulates the tyrosine phosphorylation of PDPK1 in vascular smooth muscle in a calcium- and SRC-dependent manner. Phosphorylated on Tyr-9, Tyr-373 and Tyr-376 by INSR in response to insulin. Palmitate negatively regulates autophosphorylation at Ser-241 and palmitate-induced phosphorylation at Ser-529 and Ser-501 by PKC/PRKCQ negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylation at Thr-354 by MELK partially inhibits kinase activity, the inhibition is cooperatively enhanced by phosphorylation at Ser-394 and Ser-398 by MAP3K5.Autophosphorylated; autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2.Monoubiquitinated in the kinase domain, deubiquitinated by USP4.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	O15530