Rabbit Anti-MAPK1/3 Polyclonal Antibody #abs130584

abs130584-50ug

1-2 Weeks

201

abs130584-100ug

1-2 Weeks

301

Description

This particular gene encodes a protein that belongs to the MAP kinase family. Also known as extracellular signal-regulated kinases (ERKs), MAP kinases act as a central point for multiple biochemical signals and play a crucial role in a wide range of cellular processes such as cell growth, differentiation, transcription regulation, and development. It requires phosphorylation by upstream kinases for its activation, which then leads to its translocation to the nucleus of the stimulated cells where it phosphorylates nuclear targets. There are two reported alternatively spliced transcript variants of this gene that encode the same protein but display differences in their UTRs. [provided by RefSeq]. To generate highly similar content, the text information from the original text has been rearranged.

Other names

There are a variety of names used to refer to extracellular signal-regulated kinase 1 and extracellular signal-regulated kinase 2, which are also known as ERK 1 and ERK 2. These proteins are also referred to as ERT1 and ERT2, respectively. Additionally, they may be called mitogen-activated protein kinase 1 and 2, or MAP kinase 1 and 2. Other names for ERK 1 include MAP kinase 3, p44-ERK1, and p44-MAPK, while other names for ERK 2 include p42-MAPK and p42MAPK.
These proteins are important components of the MAP kinase signaling pathway, and they are involved in a variety of cellular processes such as cell proliferation, differentiation, and survival. They are also key players in intracellular signaling cascades that are activated by various receptors, including growth factor receptors and G protein-coupled receptors.
Overall, ERK 1 and ERK 2 play crucial roles in cellular signaling, and their various names reflect their importance and widespread use in scientific research and clinical applications.

Source

Rabbit

Specificity

The detection of MAPK1/3 Antibody is aimed at identifying and measuring the naturally occurring levels of MAPK1/3. To provide a variant content, the emphasis is on rearranging the existing information in a different format.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig

Application

Western Blot (WB) is typically performed at dilutions ranging from 1:500 to 1:2000, while Immunofluorescence (IF) or Immunocytochemistry (ICC) protocols generally use dilutions of 1:100 to 1:500. On the other hand, ELISA assays using peptides as targets usually require dilutions between 1:20000 and 1:40000. These dilutions are commonly used in various experimental settings to achieve optimal results and minimize background signals.

Immunogen

A synthesized peptide derived from human MAPK1/3.

Properties

Concentration

1mg/ml

Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.

Clonality

Polyclonal Antibody

Stability & Storage

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).

Posttranslational modification

Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs.ISGylated.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Extracellular region or secreted;Golgi apparatus;Lysosome;Mitochondrion;Nucleus;Plasma Membrane;

UniPort

P28482/P27361