Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-LCP2 Polyclonal Antibody#abs136540

Kindly be informed that the aforementioned price is only for reference purposes, for more detailed and accurate pricing information, please do not hesitate to contact our seller, Vecent. Western blot analysis of extracts of various celllines, using LCP2 antibody. This product is for research use...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136540-100ug	1-2 Weeks	301.0
abs136540-50ug	1-2 Weeks	201.0

Kindly be informed that the aforementioned price is only for reference purposes, for more detailed and accurate pricing information, please do not hesitate to contact our seller, Vecent.

Overview
catalog	abs136540
Description	SLP-76 was initially identified as a ZAP-70 protein tyrosine kinase substrate after T cell receptor (TCR) engagement in Jurkat leukemic T cells. The SLP-76 gene locus is localized on chromosome 5q33 in humans, and partially characterized in mice. The human and murine cDNAs both code for 533 amino acid proteins that share 72% identity and consist of three modular domains. The N-terminal domain contains an acidic region with a PEST domain and several phosphorylated tyrosine residues following TCR engagement. SLP-76 also features a central proline-rich domain and a C-terminal SH2 domain. Other proteins have been identified that bind to SLP-76 constitutively or after receptor engagement, indicating that SLP-76 acts as an adaptor or scaffold molecule. Experiments using SLP-76 deficient T cell lines or mice have shown that SLP-76 has a positive impact on T cell development and activation, as well as mast cell and platelet function.
Other names	The 76 kDa tyrosine phosphoprotein, also known as SH2 domain-containing leukocyte protein of 76 kDa or SLP-76 tyrosine phosphoprotein, is denoted by various names including CG8697, LCP 2, LCP2, LCP2_HUMAN, and SLP76. As a lymphocyte cytosolic protein, SLP-76 is crucial for activating T cells and generating an immune response. Its SH2 domain binds to various signaling molecules and stimulates downstream pathways that regulate cytokine production and cell proliferation. SLP-76 is essential for the development and activation of T cells and its dysfunction can lead to immunodeficiency. A deeper understanding of SLP-76's role in immunity can aid in the development of therapeutic targets for autoimmune and infectious diseases.
Source	Rabbit
Specificity	The LCP2 Antibody is specifically designed to recognize and detect the total levels of endogenous LCP2 protein.
Species Reactivity	Human;Mouse;Rat
Antigen	LCP2
Application	ELISA (peptide) has a suggested dilution range of 1:20000-1:40000. For Western blotting, the recommended dilution is 1:500-1:2000. Immunohistochemistry (IHC) should be performed at dilutions ranging from 1:50-1:200. On the other hand, for immunofluorescence (IF) and immunocytochemistry (ICC), the dilution range is 1:100-1:500. It is important to adhere to these dilution guidelines to ensure accurate and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human LCP2.
MW	60kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in T-cell antigen receptor mediated signaling.
Tissue specificity	Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Posttranslational modification	Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.
Celluar localization	Cytosol;Plasma Membrane;
UniPort	Q13094