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Rabbit Anti-GM130 Polyclonal Antibody#abs137037

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Description

Catalog-specification	Delivery time	USD price
abs137037-100ug	1-2 Weeks	301.0
abs137037-50ug	1-2 Weeks	201.0

Overview
catalog	abs137037
Other names	GM130, also known as Golgi matrix protein or golgin A2, is a 130 kDa protein located in the cis-Golgi matrix. It is an autoantigen that plays a crucial role in maintaining the structure and function of the Golgi apparatus. GM130, encoded by the Golga2 gene, is a member of the golgin subfamily A. It is also referred to as Golgin-95 or SY11 protein. Another name for GM130 is Golgi autoantigen golgin subfamily a 2. The MGC20672 gene is associated with the production of GM130 protein.
Source	Rabbit
Specificity	The GM130 Antibody is specifically designed to recognize and bind to the endogenous levels of total GM130 protein. By utilizing this antibody, researchers can accurately detect and study the presence of GM130 in biological samples. It provides a valuable tool for investigating the function and localization of GM130 in various cellular processes. The GM130 Antibody is highly sensitive and reliable, ensuring accurate results in experimental studies.
Species Reactivity	Human;Mouse;Rat
Antigen	GM130
Application	，：。Western Blot（），1:10001:3000。（IHC），1:200。ELISA（），1:200001:40000。，。
Immunogen	A synthesized peptide derived from human GM130.
MW	130 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to keep it stored at a temperature of -20°C for a year without exposing it to repeated freeze/thaw cycles. It is important to avoid any temperature fluctuations that may compromise the effectiveness of the product. Taking these precautions will ensure that the product remains viable and of high quality for an extended period of time.
Storage buffer	Rabbit IgG is suspended in phosphate-buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This solution should be stored at -20 °C. It remains stable for 12 months from the date of receipt.
Target
Background	Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
Posttranslational modification	Cleaved by caspases at the onset of apoptosis.Methylation by PRMT5 is required for Golgi ribbon formation. While dimethylation at Arg-30 and Arg-35 are confirmed in vivo, it is unclear whether Arg-18 is methylated in vivo.Phosphorylated at Ser-37 by CDK1 at the onset of mitosis, inhibiting the interaction with p115/USO1 and triggering Golgi disassembly (PubMed:20421892, PubMed:26165940). Phosphorylated at Ser-37 in prophase as the Golgi complex starts to break down, and remains phosphorylated during further breakdown and partitioning of the Golgi fragments in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble (By similarity).
Celluar localization	Cytoskeleton;Golgi apparatus;Nucleus;
UniPort	Q08379