Rabbit Anti-Caspase4 Polyclonal Antibody#abs131207

abs131207-50ug

1-2 Weeks

201

abs131207-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The endogenous levels of total Caspase 4 can be detected with the Caspase 4 Antibody.

Application

The recommended dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, and for ELISA (peptide) are 1:20000-1:40000. It is important to follow these dilution guidelines to ensure accurate results.

A peptide that has been synthesized is derived from human Caspase 4. It has been produced to closely resemble the original structure and characteristics of the protein. This synthetic peptide has potential applications in a range of biological and medical fields, particularly in studying the function and activity of Caspase 4 itself. Researchers can use this peptide to gain insights into the biological processes regulated by Caspase 4 and its potential therapeutic targets. As a result, this synthesized peptide could have significant implications for future medical treatments that target Caspase 4 and related cellular pathways.

Properties

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, the antiserum was purified through peptide affinity chromatography. To achieve this, we utilized our advanced chromatography techniques to isolate and isolate the desired compounds. The result is a highly purified antiserum that is free of impurities and ready for use in various applications.

Clonality

Polyclonal Antibody

The storage temperature required to maintain the quality of the product is -20 °C for a period of one year. It is important to avoid subjecting the product to multiple instances of freezing and thawing.

Storage buffer

This is a description of Rabbit IgG, which can be stored at -20 °C in phosphate buffered saline at pH 7.4, with 150mM NaCl, 0.02% sodium azide and 50% glycerol. This product is stable for 12 months from the date of receipt.

Target

Background

Caspase-11 (also known as inflammatory caspase) is a crucial component of the NLRP3 inflammasome, which is responsible for activating CASP1 and inducing the secretion of IL1B and IL18. It is primarily activated by non-canonical triggers such as UVB radiation, cholera enterotoxin subunit B, and cytosolic LPS.
Aside from its role in the NLRP3 inflammasome, caspase-11 also promotes pyroptosis (a form of cell death) and the release of IL1A, IL18, and HMGB1 in response to non-canonical inflammasome activators. This process involves the cleavage and activation of GSDMD.
Furthermore, caspase-11 plays a critical role in limiting the replication of Salmonella typhimurium in colonic epithelial cells during infection. In the later stages of the infection, cytosolic Salmonella LPS triggers caspase-11 activation, leading to pyroptosis of infected cells, release of IL18, and extrusion of infected cells into the gut lumen. This process helps restrict bacterial replication and promotes mucosal inflammation by triggering cytokine secretion and immune cell activation.
Additionally, caspase-11 is involved in LPS-induced IL6 secretion, which may not necessarily require its enzymatic activity. It is also implicated in cell death induced by endoplasmic reticulum stress and by cytotoxic APP peptides found in Alzheimer's patient brains.
Caspase-11 can be directly activated by binding to LPS without the need for an upstream sensor. It does not directly process IL1B but is involved in the regulation of antiviral innate immune activation through the cleavage of MB21D1.

Tissue specificity

Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.

Celluar localization

Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Mitochondrion;Plasma Membrane;

P49662