Phospho-CDK2 (Thr160) Rabbit Polyclonal Antibody#abs130864

abs130864-50ug

1-2 Weeks

201

abs130864-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The Phospho-CDK2 (Thr160) Antibody specifically recognizes CDK2 that has been phosphorylated at Threonine 160. This antibody is able to detect endogenous levels of CDK2 only when it is in this phosphorylated state. Therefore, it is an important tool for researchers who are interested in studying the regulation and function of CDK2. By using this antibody, scientists can gain a better understanding of the cellular processes that are influenced by CDK2 and its phosphorylation status.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Pig;Dog;Horse;Bovine;Sheep;

Application

The recommended dilutions for using this antibody in different applications are as follows: For Western blotting, dilute the antibody 1:500-1:2000; for immunohistochemistry, dilute the antibody 1:50-1:200; for immunofluorescence or immunocytochemistry, dilute the antibody to a concentration of 1:100-1:500. For ELISA using a peptide substrate, the antibody should be diluted to a concentration of 1:20000-1:40000. These dilution ratios ensure optimal results and high sensitivity in detecting the target protein.

CDK2160。，。

Properties

1mg/ml

Purification

The purified rabbit serum produced the antibody, which was subjected to sequential chromatography on phospho- and non-phospho-peptide affinity columns using affinity purification. A highly similar content can be generated by rearranging the given information and presenting it in a different way.

Clonality

Polyclonal Antibody

To maintain its quality, store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freezing and thawing cycles.

Storage buffer

This product contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and is stable for up to 12 months from the date of receipt. Please ensure to handle with care and follow all instructions regarding storage and usage.

Target

Background

CDK2 is a serine/threonine-protein kinase that plays a crucial role in controlling the cell cycle. It is essential for meiosis, but not necessary for mitosis. CDK2 phosphorylates various proteins including CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Its activities include triggering the duplication of centrosomes and DNA, promoting the E2F transcriptional program and initiation of DNA synthesis at the G1-S transition, and modulating G2 progression. CDK2 also controls the timing of entry into mitosis and meiosis by phosphorylating and activating cyclin B/CDK1. It coordinates the activation of cyclin B/CDK1 at the centrosome and nucleus. In human embryonic stem cells, CDK2 plays a crucial role in maintaining a balance between cellular proliferation, cell death, and DNA repair. CDK2 is most active during S phase and G2, and its activity is regulated by cyclin E during the early stages of DNA synthesis and cyclin A2 during the late stages of DNA replication. CDK2 phosphorylation of EZH2 promotes the maintenance of the H3K27me3 epigenetic mark and gene silencing. It also phosphorylates CABLES1. Furthermore, CDK2 prevents oxidative stress-induced senescence by phosphorylating MYC and participates in the G1-S phase DNA damage checkpoint to prevent cells with damaged DNA from entering mitosis. CDK2 phosphorylates BRCA2, which is involved in homologous recombination-dependent DNA repair. CDK2 phosphorylation of RB1 disrupts its interaction with E2F1. It also phosphorylates NPM1, promoting its dissociation from unduplicated centrosomes and initiating centrosome duplication. CDK2-mediated phosphorylation of NPAT stimulates histone gene transcription during S phase. In addition, CDK2 is involved in vitamin D-mediated growth inhibition and nitric oxide-mediated signaling. It negatively regulates the transcriptional activity and protein stability of FOXP3 by phosphorylating it. CDK2 also phosphorylates CDK2AP2.

Tissue specificity

Induced transiently by TGFB1 at an early phase of TGFB1-mediated apoptosis.

Celluar localization

Cytoskeleton;Cytosol;Endosome;Nucleus;

P24941