Beta Actin Antibody #abs132001

abs132001-50ug

In Stock

201

abs132001-100ug

In Stock

301

Description

Actin is a crucial eukaryotic protein that serves as the fundamental building block of the cytoskeleton. In mammals, there are several isoforms of actin, with at least six known. Among these, nonmuscle β- and γ-actin, also known as cytoplasmic actin, are expressed predominantly in nonmuscle cells, where they play a critical role in controlling cell structure and motility.

Source

Rabbit

Specificity

The Beta actin antibody is capable of identifying total Beta actin levels that occur naturally within the body. To create a similar statement, the content could be rearranged and presented as follows: The endogenous levels of total Beta actin can be detected with the Beta actin antibody. This antibody accurately identifies the presence of Beta actin levels that occur naturally within the body.

Species Reactivity

Human;Mouse;Rat;Pig;Zebrafish;Dog;Monkey;Fish

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

The recommended dilutions for Western blot (WB) range from 1:3000 to 1:20000, while for Immunohistochemistry (IHC) it is 1:200. In the case of Immunofluorescence/Immunocytochemistry (IF/ICC), the suggested dilution range is 1:100 to 1:500. For ELISA with peptide antigens, a dilution of 1:20000 to 1:40000 is recommended. Please note that the provided information is unchanged and only rearranged in a similar manner.

Immunogen

The peptide derived from human Beta actin undergoes synthesis to produce a new and highly similar compound. The process involves rearranging the original content to maintain the information provided.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was used to purify the antiserum through peptide affinity chromatography. The process involved isolating and extracting the target peptides from the serum using the resin. The resulting purified antiserum was then ready for use in various applications. This technique is known for its high specificity and efficiency in separating peptides, making it a popular method of choice for antibody purification.

Clonality

Polyclonal Antibody

Stability & Storage

Ensure to store at -20 °C for one year without subjecting it to repeated freeze/thaw cycles. It is crucial to avoid freezing and thawing the product multiple times.

Storage buffer

To ensure the stability and quality of Rabbit IgG, it is recommended to store it at -20 °C in a solution containing phosphate buffered saline (pH 7.4), 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This formulation allows for long-term storage, maintaining the integrity of the antibody for up to 12 months from the date of receipt. By following these storage instructions, you can maximize the effectiveness and lifespan of the Rabbit IgG.

Target

Background

Actins are found in all eukaryotic cells and are important for cell movements. They are highly conserved proteins involved in various types of cellular motion. Actins are expressed throughout the body.

Posttranslational modification

ISGylated.Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

UniPort

P60709