Penicillin Streptomycin Solution (100 × Double Antibody)

Penicillin-streptomycin solution (100×) is a specialized antibiotic blend commonly utilized in cell cultures. After undergoing filtration and sterilization, it can be directly added to the cell culture medium. This solution comprises penicillin and streptomycin at concentrations of 10kU/mL and 10mg/mL, respectively. To prepare the solution, 0.9% sodium chloride is used as a diluent.
When incorporating this solution into cell culture medium, it is recommended to maintain working concentrations of 100U/mL for penicillin and 0.1mg/mL for streptomycin. Consequently, the solution can be diluted by a factor of 100. With a 100mL penicillin-streptomycin solution (100×), one can prepare 10L of cell culture medium.
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Adding penicillin-streptomycin solution (100× double-antibiotic) directly into a sterile cell culture medium is a common practice in laboratories. This is done to prevent bacterial contamination and maintain the sterility of the culture. The penicillin-streptomycin solution, which contains a combination of antibiotics, helps inhibit the growth of bacteria in the medium. By introducing the antibiotics directly into the culture medium, the risk of bacterial contamination can be significantly reduced, allowing researchers to focus on their experiments without worrying about unwanted microbial growth.
To prepare your cell culture medium, simply add 5ml of penicillin-streptomycin solution (100× double-antibiotic) to 500ml of medium. Be sure to mix these ingredients thoroughly before using. This will help ensure that your cell culture is free from contamination while promoting healthy growth.
Filter and sterilize the cell culture medium after adding the penicillin-streptomycin solution (100× double-antibiotic) during preparation.
To prepare the cell culture solution, you should incorporate a 100× double-antibiotic, specifically the penicillin-streptomycin solution, in a proportion of 10 mL for every 1L of the cell culture solution. Furthermore, it is necessary to filter and sterilize the solution subsequent to its preparation before using it for your experiments.

Store at -20° C for 12 months.

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Penicillin-streptomycin solution (100× secondary antibiotic)