GelRed Nucleic Acid Dyes

 Characteristics of Gelred Nucleic Acid Dyes: 1.     Non-toxic: The unique characteristics of oil and large molecular weight of Gelred make it unable to penetrate the cell membrane and enter the cell. The results of Ames test also showed that the mutagenicity of this dye is far less than that of EB.2.      High sensitivity: suitable for electrophoretic staining of various size fragments, the effect on nucleic acid migration is less than that of SYBR Green I.3.      High stability: suitable for preparing agarose gel by microwave or other heating methods;Extremely stable in acid or alkali buffers at room temperature, with excellent light resistance.4.      High signal-to-noise ratio: sample fluorescence signal is strong, background signal is low.5.      Easy to operate: like EB, the dye does not degrade during prefabrication and electrophoresis;After electrophoresis, the dyeing process takes only 30min without decolorization or rinsing, and can be observed directly with UV gel transmitters.6.      Wide range of application: pre-electrophoresis dyeing (gel dyeing method) or post-electrophoresis dyeing (foam dyeing method) can be selected;Suitable for agarose gel or polyacrylamide gel electrophoresis;Can be used for dsDNA, ssDNA or RNA staining.7.      It has the same spectral characteristics as EB without changing the filter and observation position: standard EB filter or SYBR filter is suitable for observation, using the same common UV gel transmitometer as the observation of EB, the best excitation can be obtained near the UV light of 300nm.However, GELRED cannot be fully lasered by a 488nm argon ion laser or visible light of similar wavelength, so the imaging system with such excitation device is not recommended.For this type of device, we recommend that you use SuperGreen, which has a similar spectrum to the SYBR Green I, with the same sensitivity but more stability. Usage: 1.      Rubber Dyeing Method (Usage Same as EB) (Recommended Method)     Gelred nucleic acid dye is added to the gelatin (e.g., 5ul Gelred 10000* storage solution for every 50ml agarose solution, and so on).b.      Electrophoresis is performed as usual.  Note: the amount of dye used in this method is relatively small.500ul can make about 100 pieces of 500ml glue.Because of its good thermal stability, GelRed can be added directly to hot agarose solutions without waiting for the solution to cool.Shake, oscillate, or flip to make sure the dye mixes well.Alternatively, the GELRED storage solution can be added to the agarose powder and the electrophoretic buffer and then heated in the microwave oven or in its usual manner to prepare the agarose gel. GELRED is compatible with all common electrophoretic buffers.If the bands are consistently seen to be diffused or separated unsatisfyingly, a bubble staining method is recommended to confirm whether the problem is dye related.If the problem still exists after dyeing, it means the problem has no light with the dye. Please try: reduce the concentration of agarose and choose a longer gel;Lengthen the gel time to ensure clear edges;Improve sample loading technique or select foam dyeing method.This method is not suitable for precast polyacrylamide gel. For polyacrylamide gel, please use the foam dyeing method.2.      Bubble staining a. & have spent     The electrophoresis was performed by conventional methods.     Dilute Gelred nucleic acid dye (10000*H2O) storage solution in water approximately 3300 times to 0.1M NaCl to make 3* staining solution (for example, add 15ul Gelred nucleic acid dye (10000*H2O) storage solution and 5ml 1M NaCl to 45ml H2O).     Place the gel carefully in a suitable container, such as polypropylene.Slowly add enough 3* staining solution to immerse the gel.The optimal staining time was slightly different according to the gel thickness and the concentration of agarose.For gels containing 3.5% to 10% acrylamide, the dyeing time was usually between 30min and 1h and increased with increasing acrylamide content.Note: the amount of dye is more when the dye is soaked.The 3* Gelred stain can be prepared in large quantities and stored at room temperature, away from light, until used up.Special note: if you are using UV imager, please select Gelred;If you use a laser imager or wish to observe in visible light, select SuperGreen.In rare cases where the DNA sample after the plasmid is digested by certain enzymes shows trailing and reduced resolution, it is recommended to try both staining methods to determine which is more appropriate. Storage: -20° C away from light, expiry date: 1 year.

Store at 4° C and avoid light for 1 year.