Glycogen Synthase Microplate Assay Kit

Glycogen (starch) synthetase is also known as EC 2.4.1.11 or UDP-glucose-glycogen glucosyltransferase. It is an important enzyme that catalyzes the transfer of glucose molecules from UDP-glucose to glycogen, ultimately leading to the formation of glycogen. Other names for this enzyme include UDP-glycogen synthase, UDPG-glycogen synthetase, UDPG-glycogen transglucosylase, and uridine diphosphoglucose-glycogen glucosyltransferase. It plays a vital role in carbohydrate metabolism by converting glucose into a readily available energy source for the body. Understanding the function and regulation of glycogen synthetase is essential for the management of diseases such as diabetes and glycogen storage diseases.

The activity of glycogen synthase can be detected and quantified using various methods. This process involves determining the level of glycogen synthase activity in a given sample.
To detect and quantify glycogen synthase activity, researchers typically employ enzymatic assays. These assays utilize specific substrates and enzymes to measure the conversion of glucose molecules into glycogen by glycogen synthase.
One commonly used method for detecting glycogen synthase activity is the radioactive assay. In this assay, a radioisotope-labeled glucose molecule is provided as a substrate, and its incorporation into glycogen by glycogen synthase is measured.
Another method is the colorimetric assay, which relies on the enzymatic reaction between glucose molecules and a chromogenic reagent. This reaction produces a colored compound that can be detected using a spectrophotometer. The intensity of the color is proportional to the amount of glycogen synthase activity present in the sample.
Furthermore, researchers can also employ Western blotting techniques to quantify the protein expression levels of glycogen synthase in a sample. This method involves separating proteins based on their molecular weight using gel electrophoresis and transferring them onto a membrane. The membrane is then probed with specific antibodies against glycogen synthase, and the resulting signal can be quantified.
Overall, the detection and quantification of glycogen synthase activity are important for understanding its role in glycogen metabolism and its potential implications in various diseases such as diabetes and glycogen storage disorders.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.