Glutamate Dehydrogenase Microplate Assay Kit

Glutamate dehydrogenase, also known as glutamic dehydrogenase or GLDH, is an enzyme with the EC number 1.4.1.2. Its main function is to catalyze the conversion of glutamate to alpha-ketoglutarate. This enzymatic reaction is essential for the proper metabolism of glutamate in various biological processes. Glutamate dehydrogenase plays a crucial role in nitrogen metabolism and is found in many organisms, including bacteria, plants, and animals. Its activity is regulated by various factors, such as the levels of substrates and products, as well as the presence of allosteric effectors. Understanding the structure and function of glutamate dehydrogenase is important for studying its role in cellular metabolism and developing potential therapeutic interventions targeted at this enzyme.

The activity of glutamate dehydrogenase can be detected and quantified through various methods. These methods aim to measure the conversion of glutamate to alpha-ketoglutarate and ammonia which is the key reaction of this enzyme. By measuring the rate of this conversion, the activity of glutamate dehydrogenase can be determined. Reliable and sensitive assays have been developed that use spectrophotometric, radiometric, and colorimetric techniques to analyze the enzyme activity. Such methods play a crucial role in the diagnosis of certain metabolic disorders and also in the understanding of the enzyme’s role in various biological processes.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.