Alpha-Ketoglutarate Dehydrogenase Microplate Assay Kit

The enzyme known as EC 1.2.4.2, or 2-ketoglutarate dehydrogenase, also goes by several other names including AKGDH, alpha-ketoglutaric acid dehydrogenase, and oxoglutarate dehydrogenase. Its primary function is to catalyze the conversion of 2-oxoglutarate (also known as alpha-ketoglutarate) into succinyl-CoA in the tricarboxylic acid (TCA) cycle. This process involves the removal of a carbon atom from 2-oxoglutarate and the transfer of electrons to NAD+, forming NADH. The enzyme requires several cofactors including thiamine pyrophosphate, lipoic acid, and FAD. mutations in the genes encoding this enzyme can lead to a variety of metabolic disorders.

Alpha-ketoglutarate dehydrogenase activity can be detected and quantified using various methods. This enzyme plays a crucial role in the citric acid cycle and is responsible for converting alpha-ketoglutarate into succinyl-CoA. One common approach to measure this activity is by monitoring the production of NADH, which is coupled to the reaction catalyzed by alpha-ketoglutarate dehydrogenase. Another method involves the use of radioisotopes or fluorescent probes to directly measure the conversion of alpha-ketoglutarate into succinyl-CoA. These techniques provide valuable insights into the functioning of alpha-ketoglutarate dehydrogenase and its involvement in cellular metabolism. Additionally, the quantification of its activity is essential for studying diseases associated with dysfunctional alpha-ketoglutarate dehydrogenase, such as neurodegenerative disorders and metabolic diseases.

4 umol/L - 400 umol/L

Shipped and store at 4°C for 6 months.